Martin C, Prescott A, Mackay S, Bartlett J, Vrijlandt E
Department of Genetics, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, UK.
Plant J. 1991 Jul;1(1):37-49. doi: 10.1111/j.1365-313x.1991.00037.x.
The intensity and pattern of anthocyanin biosynthesis in Antirrhinum flowers is controlled by several genes. We have isolated six cDNA clones encoding enzymes in the pathway committed to flavonoid biosynthesis and used these to assay how the regulatory genes that modify colour pattern affect the expression of biosynthetic genes. The biosynthetic genes of the later part of the pathway appear to be co-ordinately regulated by two genes, Delila (Del), and Eluta (El), while the early steps (which also lead to flavone synthesis) are controlled differently. This division of control is not the same as control of anthocyanin biosynthesis by the regulatory genes R (S) and C1 in maize aleurone, and may result from the adaptive significance of different flavonoids in flowers and seeds, reflecting their attractiveness to insects and mammals respectively. El and del are probably involved in transcriptional control and both genes appear to be able to repress expression of some biosynthetic genes and activate expression of others.
金鱼草花中花青素生物合成的强度和模式受多个基因控制。我们分离出了六个编码参与类黄酮生物合成途径中酶的cDNA克隆，并利用这些克隆来分析改变花色模式的调控基因如何影响生物合成基因的表达。该途径后期的生物合成基因似乎受两个基因，即Delila（Del）和Eluta（El）的协同调控，而早期步骤（也导致黄酮合成）则受到不同的控制。这种控制的划分与玉米糊粉层中调控基因R（S）和C1对花青素生物合成的控制不同，这可能是由于花和种子中不同类黄酮的适应性意义所致，分别反映了它们对昆虫和哺乳动物的吸引力。El和del可能参与转录调控，并且这两个基因似乎都能够抑制一些生物合成基因的表达并激活其他基因的表达。
