National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
Kazusa DNA Research Institute, 2-6-7 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan.
Sci Rep. 2023 Oct 17;13(1):17674. doi: 10.1038/s41598-023-44404-z.
Recently, many new cultivars have been taken abroad illegally, which is now considered an international issue. Botanical evidence found at a crime scene provides valuable information about the origin of the sample. However, botanical resources for forensic evidence remain underutilized because molecular markers, such as microsatellites, are not available without a limited set of species. Multiplexed intersimple sequence repeat (ISSR) genotyping by sequencing (MIG-seq) and its analysis method, identification of not applicable (iD-NA), have been used to determine several genome-wide genetic markers, making them applicable to all plant species, including those with limited available genetic information. Camellia cultivars are popular worldwide and are often planted in many gardens and bred to make new cultivars. In this study, we aimed to analyze Camellia cultivars/species through MIG-seq. MIG-seq could discriminate similar samples, such as bud mutants and closely related samples that could not be distinguished based on morphological features. This discrimination was consistent with that of a previous study that classified cultivars based on short tandem repeat (STR) markers, indicating that MIG-seq has the same or higher discrimination ability as STR markers. Furthermore, we observed unknown phylogenetic relationships. Because MIG-seq can be applied to unlimited species and low-quality DNA, it may be useful in various scientific fields.
最近，许多新的栽培品种被非法带出国境，这现在被认为是一个国际问题。在犯罪现场发现的植物学证据为样本的起源提供了有价值的信息。然而，由于没有有限的物种集合，分子标记（如微卫星）不可用，因此法医证据的植物资源仍未得到充分利用。通过测序的多重简单重复序列（ISSR）基因分型（MIG-seq）及其分析方法，即不可用识别（iD-NA），已被用于确定几个全基因组遗传标记，使其适用于所有植物物种，包括那些遗传信息有限的物种。茶花品种在世界范围内广受欢迎，经常种植在许多花园中，并被培育成新的品种。在这项研究中，我们旨在通过 MIG-seq 分析茶花品种/物种。MIG-seq 可以区分相似的样本，例如芽突变体和基于形态特征无法区分的密切相关的样本。这种区分与之前基于短串联重复（STR）标记对品种进行分类的研究一致，表明 MIG-seq 具有与 STR 标记相同或更高的区分能力。此外，我们观察到了未知的系统发育关系。由于 MIG-seq 可以应用于无限的物种和低质量的 DNA，它可能在各种科学领域都有用。
