Nirala Satendra Kumar, Bhadauria Monika
Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior (M.P.), India.
Arch Pharm Res. 2008 Apr;31(4):451-61. doi: 10.1007/s12272-001-1178-5. Epub 2008 May 1.
The present study has been conducted to evaluate the curative effect of propolis extract, a honey bee-hive product, against acetaminophen (APAP) induced oxidative stress and dysfunction in liver and kidney. Animals were challenged with APAP (2 g/kg, p.o.) followed by treatment of propolis extract (100 and 200 mg/kg, p.o.) once only after 24 h. Release of transaminases, alkaline phosphatase, lactate dehydrogenase, and serum bilirubin were increased, whereas hemoglobin and blood sugar were decreased after APAP administration. Antioxidant status in both the liver and kidney tissues were estimated by determining the glutathione, malondialdehyde content and activities of the CYP enzymes, which showed significant alterations after APAP intoxication. In addition, activities of adenosine triphosphatase, acid phosphatase, alkaline phosphatase, and major cell contents (total protein, glycogen and cholesterol) were also altered due to APAP poisoning. Propolis extract successfully reversed the alterations of these biochemical variables at higher dose. Improvements in hepatorenal histoarchitecture were also consistent with biochemical observations. The results indicated that ethanolic extract of propolis has ability to reverse APAP-induced hepatorenal biochemical and histopathological alterations probably by increasing the antioxidative defense activities due to various phenolic compounds present in it.
本研究旨在评估蜂胶提取物（一种蜂巢产品）对乙酰氨基酚（APAP）诱导的肝脏和肾脏氧化应激及功能障碍的治疗效果。动物经APAP（2 g/kg，口服）攻击，24小时后仅一次给予蜂胶提取物（100和200 mg/kg，口服）。给予APAP后，转氨酶、碱性磷酸酶、乳酸脱氢酶和血清胆红素的释放增加，而血红蛋白和血糖降低。通过测定谷胱甘肽、丙二醛含量和CYP酶活性来评估肝脏和肾脏组织中的抗氧化状态，APAP中毒后这些指标显示出显著变化。此外，由于APAP中毒，三磷酸腺苷酶、酸性磷酸酶、碱性磷酸酶的活性以及主要细胞成分（总蛋白、糖原和胆固醇）也发生了改变。蜂胶提取物在较高剂量时成功逆转了这些生化变量的变化。肝肾组织结构的改善也与生化观察结果一致。结果表明，蜂胶乙醇提取物可能通过增加其中所含各种酚类化合物的抗氧化防御活性，具有逆转APAP诱导的肝肾生化和组织病理学改变的能力。
