Japan Beekeeping Co. Ltd., Gifu 500-8691, Japan.
Evid Based Complement Alternat Med. 2013;2013:302720. doi: 10.1155/2013/302720. Epub 2013 Apr 22.
We examined the protective effect of Brazilian propolis against liver damage with cholestasis in rats treated with α-naphthylisothiocyanate (ANIT) in comparison with that of vitamin E (VE). Rats orally received Brazilian propolis ethanol extract (BPEE) (25, 50, or 100 mg/kg), VE (250 mg/kg) or vehicle at 12 h after intraperitoneal injection of ANIT (75 mg/kg) and were killed 24 h after the injection. Vehicle-treated rats showed liver cell damage and cholestasis, judging from the levels of serum marker enzymes and components. The vehicle group had increased serum total cholesterol, triglyceride, phospholipid, and lipid peroxide levels, increased hepatic lipid peroxide, reduced glutathione, and ascorbic acid levels and myeloperoxidase activity, and decreased hepatic superoxide dismutase activity. BPEE (50 mg/kg) administered to ANIT-treated rats prevented liver cell damage and cholestasis and attenuated these serum and hepatic biochemical changes except hepatic ascorbic acid, although administered BPEE (25 or 100 mg/kg) was less effective. VE administered to ANIT-treated rats prevented liver cell damage, but not cholestasis, and attenuated increased serum lipid peroxide level, increased hepatic lipid peroxide level and myeloperoxidase activity, and decreased hepatic superoxide dismutase activity. These results indicate that BPEE protects against ANIT-induced liver damage with cholestasis in rats more effectively than VE.
我们研究了巴西蜂胶对α-萘异硫氰酸酯（ANIT）处理大鼠肝损伤伴胆汁淤积的保护作用，并与维生素 E（VE）进行了比较。大鼠在腹腔注射 ANIT（75mg/kg）后 12 小时，经口给予巴西蜂胶乙醇提取物（BPEE）（25、50 或 100mg/kg）、VE（250mg/kg）或载体，注射后 24 小时处死。从血清标志物酶和成分的水平来看，给予载体的大鼠表现出肝细胞损伤和胆汁淤积。与载体组相比，载体组血清总胆固醇、甘油三酯、磷脂和脂质过氧化物水平升高，肝脂质过氧化物、还原型谷胱甘肽和抗坏血酸水平降低，髓过氧化物酶活性升高，肝超氧化物歧化酶活性降低。给予 ANIT 处理大鼠 50mg/kg 的 BPEE 可预防肝细胞损伤和胆汁淤积，并减轻除肝抗坏血酸以外的这些血清和肝生化变化，尽管给予 25 或 100mg/kg 的 BPEE 效果较差。给予 ANIT 处理大鼠 VE 可预防肝细胞损伤，但不能预防胆汁淤积，并减轻血清脂质过氧化物水平升高、肝脂质过氧化物水平和髓过氧化物酶活性升高以及肝超氧化物歧化酶活性降低。这些结果表明，BPEE 对 ANIT 诱导的大鼠肝损伤伴胆汁淤积的保护作用比 VE 更有效。
