Siddiqa Ayesha, Qureshi Rahmatullah, Raja Naveed Iqbal, Khan Imtiaz Ahmed, Ahmad Muhammad Zishan, Rafique Shaista, Ali Amir, Ahmad Ajaz, Kaushik Prashant
Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Department of Veterinary Pathology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Front Pharmacol. 2024 Feb 21;15:1325359. doi: 10.3389/fphar.2024.1325359. eCollection 2024.
Liver disease is a serious health concern in today's world, posing a challenge to both healthcare providers and pharmaceutical companies. Most synthetic drugs and chemicals cause liver damage accounting for approximately 10% of acute hepatitis and 50% of acute liver failure. The present study aimed to evaluate the hepato-protective activity of an extract of chicory formulation assisted by silver nanoparticles against carbon tetra chloride (CCl)-induced hepatic damage in rat's liver. Rats of the Wistar strain (Rattus norvegicus) were used to test the hepato-protective efficacy at various doses. Rats were randomly divided into nine groups, each containing six rats. The groups were as follows: first group (control), second group (CCl), third group, silymarin (20 mg/kg of body weight), fourth group (CCl+chicory) (1.75 mg/kg of b. wt), fifth group (CCl + chicory at the dose of 2.35 mg/kg), sixth group (CCl + chicory of 3.25 mg/kg), seventh group (CCl +AgNPs 1.75 mg/kg of b. wt.), eighth group (CCl + AgNPs 2.35 mg/kg of body weight), and ninth group (CCl + AgNPs 3.25 mg/kg of b. wt.). Blood samples were taken 24 h after the last administration (i.e., 30th day). The blood samples were analyzed for different serum enzymes such as ALP , ALT (alanine transaminase), bilirubin (Blr), triglyceride, and cholesterol. Histology liver sections were performed. Treatment with AgNPs and chicory extract showed significant hepato-protective activity in a dose-dependent manner. In three doses, the chicory extract at a rate of 3.25 mg/kg of body weight significantly reduced elevated levels of biochemical markers in comparison to CCl-intoxicated rats. Histology of the liver sections from CCl-treated rats revealed inflammation of hepatocytes, necrosis, cytoplasmic degeneration, vacuolization, and a deformed central vein. The chicory formulation extract exhibited a remarkable recovery percentage in the liver architecture that was higher than the drug (i.e., silymarin). While treatment with AgNPs also repaired the degenerative changes and restored the normal form of the liver, chicory formulation extract possessed more hepato-protective potential as compared to AgNPs by regulating biochemical and histo-pathological parameters. This study can be used as confirmation of the hepato-protective potential of chicory compounds for possible use in the development programs of drugs to treat liver diseases.
在当今世界，肝脏疾病是一个严重的健康问题，对医疗服务提供者和制药公司都构成了挑战。大多数合成药物和化学物质会导致肝损伤，约占急性肝炎的10%和急性肝衰竭的50%。本研究旨在评估由银纳米颗粒辅助的菊苣制剂提取物对四氯化碳（CCl）诱导的大鼠肝脏肝损伤的保肝活性。使用Wistar品系（褐家鼠）的大鼠在不同剂量下测试保肝功效。大鼠被随机分为九组，每组六只。分组如下：第一组（对照组），第二组（CCl组），第三组，水飞蓟宾（20毫克/千克体重），第四组（CCl+菊苣）（1.75毫克/千克体重），第五组（CCl+菊苣，剂量为2.35毫克/千克），第六组（CCl+菊苣，3.25毫克/千克），第七组（CCl+银纳米颗粒，1.75毫克/千克体重），第八组（CCl+银纳米颗粒，2.35毫克/千克体重），第九组（CCl+银纳米颗粒，3.25毫克/千克体重）。在最后一次给药后24小时（即第30天）采集血样。对血样进行分析，检测不同的血清酶，如碱性磷酸酶、谷丙转氨酶（丙氨酸转氨酶）、胆红素、甘油三酯和胆固醇。进行肝脏组织学切片检查。银纳米颗粒和菊苣提取物的治疗均显示出显著的剂量依赖性保肝活性。在三个剂量水平下，与CCl中毒大鼠相比，体重为3.25毫克/千克的菊苣提取物显著降低了生化标志物的升高水平。CCl处理大鼠肝脏切片的组织学检查显示肝细胞炎症、坏死、细胞质变性、空泡化以及中央静脉变形。菊苣制剂提取物在肝脏结构中表现出显著的恢复率，高于药物（即水飞蓟宾）。虽然银纳米颗粒的治疗也修复了退行性变化并恢复了肝脏的正常形态，但通过调节生化和组织病理学参数，菊苣制剂提取物比银纳米颗粒具有更强的保肝潜力。本研究可作为菊苣化合物保肝潜力的证据，用于治疗肝脏疾病药物开发项目。
