Modulation of calcium signals by fluorescent dyes in the presence of tubular endoplasmic reticulum: a modelling approach.

The complex network of Ca(2+) signals uses local events as building blocks for generating global calcium signals with different shapes. However, the nature of the large time- and space-scales of local calcium signals observed in Xenopus oocytes has remained unclear. By numeric simulations that include optical blurring of the image and the geometrical restrictions imposed by tubules of the endoplasmic reticulum or other cell structures, we investigate how the fluorescent dye affect the observed features of calcium events, such as rate of signal decay, spatial size, fluorescence amplitude, or the apparent diffusion like from a point source in a spherically symmetric space. We add more evidence that, irrespective of the dye properties, local calcium signals produced in the presence of tubular cellular structures are consistently wider than expected in a homogeneous environment. Moreover, the spatial dimension and the decay time of the event increase with the quantity of liberated Ca(2+). Our results also indicate that a fast binding Ca(2+) indicator that does not bind to cytosolic proteins yields fast signals when the event is observed in the front of the release site, and slow signals when the event is viewed from the opposite side of the tubule. We propose several ways to test our model by various experimental procedures.