Chylińska W, Bieleń M, Bilski R, Dziaduś-Sokołowska A, Michalski J, Mroczka J, Sieppel-Maciejewska K
Zakładu Biofizyki Instytutu Fizjologii Akademii Medycznej im. M. Kopernika, Krakowie.
Folia Med Cracov. 1991;32(3-4):227-45.
In experiments performed on anaesthetized male and female Wistar rats the effects of different types of hypoxia on the electrical potential difference (PD) between the inner and the outer sides of the gastric wall were examined. The PD was determined by mean of the Digital Multimeter VC-10T, Unitra, and the calomel electrodes connected with the KCl-agar bridges. There were four series of experiments carried out in which hypoxia was produced by: I--low atmospheric pressure (hypobaric hypoxia) corresponding with the altitudes of 2500, 5500, 8500 and 10,500 m above sea level, II--1 min. nitrogen breathing (anoxic anoxia), III--bleeding ca. 1% of the body weight (anemic hypoxia), IV--the gastric vessels ligation (ischemic hypoxia). There were also performed the adequate control experiments of each series. In all types of hypoxia (I--IV) a decrease in the PD was observed. The value and rate of this decrease were dependent on the type, grade, duration and rate of hypoxia. In the conditions of the hypobaric hypoxia simulated altitude 10,500 m only evoked the statistically significant PD drops, by 31%. The nitrogen breathing caused the PD decrease by 23% and the anemic hypoxia by 18%. In the ischemic hypoxia the total disappearance of the potential difference (PD = 0 mV) was observed. In the control experiments small non significant fluctuations, not exceeding 4%, occurred only. The decrease in the PD of the gastric wall observed during hypoxia could be explained by the changes in the membrane transport e.g. the back-diffusion of Na+, Cl-, and H+ ions.
在对麻醉的雄性和雌性Wistar大鼠进行的实验中，研究了不同类型的缺氧对胃壁内外侧电势差（PD）的影响。通过Digital Multimeter VC - 10T、Unitra数字万用表以及与KCl - 琼脂桥相连的甘汞电极来测定PD。进行了四个系列的实验，其中缺氧是通过以下方式产生的：I - 对应于海拔2500米、5500米、8500米和10500米高度的低气压（低氧性缺氧）；II - 1分钟的氮气呼吸（缺氧性缺氧）；III - 失血约占体重的1%（贫血性缺氧）；IV - 胃血管结扎（缺血性缺氧）。每个系列还进行了适当的对照实验。在所有类型的缺氧（I - IV）中，均观察到PD下降。这种下降的数值和速率取决于缺氧的类型、程度、持续时间和速率。在模拟海拔10500米的低氧性缺氧条件下，仅引起了具有统计学意义的PD下降，下降了31%。氮气呼吸导致PD下降23%，贫血性缺氧导致下降18%。在缺血性缺氧中，观察到电势差完全消失（PD = 0 mV）。在对照实验中，仅出现了不超过4%的小的、无统计学意义的波动。缺氧期间观察到的胃壁PD下降可以通过膜转运的变化来解释，例如Na +、Cl - 和H + 离子的反向扩散。
