Wistar大鼠慢性脊髓损伤细胞治疗后骨髓干细胞（CD45(+)/CD34(-)）的功能结局

Functional outcome of bone marrow stem cells (CD45(+)/CD34(-)) after cell therapy in chronic spinal cord injury in Wistar rats.

作者信息

Carvalho K A T, Vialle E N, Moreira G H G, Cunha R C, Simeoni R B, Francisco J C, Guarita-Souza L C, Oliveira L, Zocche L, Olandoski M

机构信息

Instituto de Ciências Biológicas e da Saúde, da Pontificia Universidade Católica do Paraná (ICBS-PUCPR), Curitiba, Brazil.

出版信息

Transplant Proc. 2008 Apr;40(3):845-6. doi: 10.1016/j.transproceed.2008.02.054.

DOI:10.1016/j.transproceed.2008.02.054

PMID:18455033

Abstract

BACKGROUND

Therapy with diverse cell types has been proposed to regenerate spinal cord injuries seeking to minimize the consequences for the lives of chronic patients. The types considered are: mononuclear and mesenchymal adult stem cells, embryonic stem cells, and Schwann cells.

MATERIALS AND METHODS

Ninety male Wistar rats that underwent spinal cord contusion injury (NYU Impactor) were followed with the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale for 14 days. Animals with scores < or = 16 were randomly divided into 2 groups: control (vehicle) versus cell therapy group. The mononuclear fraction (CD45(+)/CD34(-)) obtained by puncture-aspiration of the bone marrow was isolated by a density gradient (d = 1.077). The parenchymal cell infusion was performed using a syringe (100 U/1 mL) with a 30G1/2 needle. The animals were followed for 10 days before euthanasia. Statistical analyses comparing groups were performed by the Mann-Whitney test and group comparisons by the Wilcoxon test.

RESULTS

Among 90 injured rats, 65 (72.2%) survived, including 44 whose scores were < or = 16. Eleven animals finished the study in the control group (64.7%) and 17 in the therapy group (80.9%). The statistical analyses did not demonstrate significance (P > .05) for either test.

CONCLUSION

Mononuclear adult stem cell therapy was not demonstrated to be functionally effective for chronic spinal cord injury.

摘要

背景

为使慢性患者的生活后果最小化，已提出用多种细胞类型进行治疗以再生脊髓损伤。所考虑的细胞类型有：单核和间充质成体干细胞、胚胎干细胞和雪旺细胞。

材料与方法

90只接受脊髓挫伤损伤（纽约大学撞击器）的雄性Wistar大鼠，采用Basso、Beattie和Bresnahan（BBB）运动评分量表随访14天。评分≤16分的动物被随机分为2组：对照组（赋形剂）和细胞治疗组。通过骨髓穿刺抽吸获得的单核部分（CD45(+)/CD34(-)）通过密度梯度（d = 1.077）分离。使用带有30G1/2针头的注射器（100 U/1 mL）进行实质细胞输注。在安乐死之前对动物随访10天。通过Mann-Whitney检验进行组间比较的统计分析，通过Wilcoxon检验进行组内比较。