Department of Pharmacology, Faculty of Pharmacy, University of Sana'a, Sana'a, Yemen.
Department of Biochemistry & Molecular Biology, Faculty of Medicine and Health Sciences, University of Sana'a, Sana'a, Yemen.
Biomed Res Int. 2020 Feb 17;2020:2425693. doi: 10.1155/2020/2425693. eCollection 2020.
The aim of this study was to investigate the anticancer and antioxidant activities as well as the safety of the brown algae of the Western seacoast of Yemen. Cytotoxicity of methanol extract of and several of its fractions, petroleum ether, chloroform, ethyl acetate, n-butanol, and aqueous extracts against seven different cancer cell lines was determined by crystal violet staining. The antioxidant activity was also assessed using the DPPH radical scavenging assay. Acute toxicity study was performed on rats at increasing doses of the methanol extract. Extracts of exerted a significant dose-dependent cytotoxicity on the seven tumor cell lines but were generally more selective on MCF-7 and PC-3. Among all fractions, the chloroform fraction of the displayed the highest cytotoxic activity and was most effective in MCF-7, PC3, and CACO cells (IC = 1.93 ± 0.25, 2.2 ± 0.18, and 2.71 ± 0.53 g/mL, respectively). The petroleum ether fraction was also effective, particularly against MCF-7 and PC-3 (IC = 4.77 ± 0.51 and 3.93 ± 0.51 g/mL, respectively) whereas the activity of the ethyl acetate fraction was more pronounced against HepG2 and CACO (IC = 5.06 ± 0.21 and 5.06 ± 0.23 g/mL, respectively). Of all the extracts tested, the crude methanolic extract of the algae exhibited only a modest antioxidant potential (IC = 204.6 ± 8.3 g/mL). Doses as high as 5000 mg/kg body weight of methanolic extracts were safe and well tolerated by rats. The overall results showed that exhibited a significant cytotoxic activity probably due to the occurrence of nonpolar cytotoxic compounds, which is independent of its antioxidant capability.
本研究旨在探究也门西部沿海地区褐藻的抗癌和抗氧化活性及其安全性。采用结晶紫染色法测定甲醇提取物及其石油醚、氯仿、乙酸乙酯、正丁醇和水提取物对七种不同癌细胞系的细胞毒性。还使用 DPPH 自由基清除 assay 评估了抗氧化活性。在大鼠身上进行了甲醇提取物递增剂量的急性毒性研究。提取物对七种肿瘤细胞系表现出显著的剂量依赖性细胞毒性，但对 MCF-7 和 PC-3 更为特异。在所有馏分中，褐藻的氯仿馏分显示出最高的细胞毒性活性，对 MCF-7、PC3 和 CACO 细胞最为有效（IC=1.93±0.25、2.2±0.18 和 2.71±0.53μg/mL）。石油醚馏分也具有活性，特别是对 MCF-7 和 PC-3（IC=4.77±0.51 和 3.93±0.51μg/mL），而乙酸乙酯馏分对 HepG2 和 CACO 的活性更为显著（IC=5.06±0.21 和 5.06±0.23μg/mL）。在所测试的所有提取物中，藻类的粗甲醇提取物仅表现出适度的抗氧化潜力（IC=204.6±8.3μg/mL）。高达 5000mg/kg 体重的藻类甲醇提取物剂量对大鼠是安全且耐受良好的。总的来说，结果表明褐藻表现出显著的细胞毒性活性，可能是由于存在非极性细胞毒性化合物，这与其抗氧化能力无关。
