Alexander J, Bey E, Whitcutt J M, Gear J H
S Afr J Med Sci. 1976;41(2):89-98.
Forty-five human malignant tumour specimens were cultivated in vitro in an attempt to determine the necessary conditions for tumour cell maintenance and to establish permanently-growing cell lines. Continuously-growing cultures were derived from five tumours, including carcinomas of the oesophagus and colon, a hepatoma, a mesothelioma and a retroperitoneal sarcoma. The carcinoma of the oesophagus and the hepatoma, which have adapted fully to in vitro conditions, can be regarded as established cell lines.
为了确定肿瘤细胞维持生长的必要条件并建立永久生长的细胞系，对45份人类恶性肿瘤标本进行了体外培养。连续生长的培养物源自5种肿瘤，包括食管癌、结肠癌、肝癌、间皮瘤和腹膜后肉瘤。已经完全适应体外条件的食管癌和肝癌可被视为已建立的细胞系。
