• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用环介导等温扩增技术（LAMP）快速检测食品中的 。

Rapid Detection of in Food Using Loop-Mediated Isothermal Amplification (LAMP).

机构信息

School of Grain Science and Technology, Jilin Business and Technology College, Changchun 130507, China.

College of Food Engineering, Jilin Engineering Normal University, Changchun 130052, China.

出版信息

Int J Environ Res Public Health. 2021 Apr 21;18(9):4401. doi: 10.3390/ijerph18094401.

DOI:10.3390/ijerph18094401
PMID:33919101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8122632/
Abstract

Botulinum neurotoxins are considered as one of the most potent toxins and are produced by . It is crucial to have a rapid and sensitive method to detect the bacterium in food. In this study, a rapid detection assay of in food using loop-mediated isothermal amplification (LAMP) technology was developed. The optimal primers were identified among three sets of primers designed specifically based on the partial gene encoding nontoxic-nonhaemagglutinin (NTNH) for rapid detection of the target DNA in plasmids. The optimal temperature and reaction time of the LAMP assay were determined to be 64 °C and 60 min, respectively. The chemical kit could be assembled based on these optimized reaction conditions for quick, initial high-throughput screening of in food samples. The established LAMP assay showed high specificity and sensitivity in detecting the target DNA with a limit of 0.0001 pg/ul (i.e., ten times more sensitive than that of the PCR method) and an accuracy rate of 100%. This study demonstrated a potentially rapid, cost-effective, and easy-operating method to detect in food and clinical samples based on LAMP technology.

摘要

肉毒杆菌毒素被认为是最有效的毒素之一，由 产生。快速灵敏的方法来检测食品中的 细菌至关重要。本研究利用环介导等温扩增（LAMP）技术开发了一种快速检测食品中 的方法。从三组专门根据编码非毒性非血凝素（NTNH）的部分 基因设计的引物中鉴定出最佳引物，用于快速检测质粒中的靶 DNA。LAMP 反应的最佳温度和反应时间分别确定为 64°C 和 60 分钟。可以根据这些优化的反应条件组装化学试剂盒，用于快速、初步高通量筛选食品样品中的 。建立的 LAMP 检测方法具有高度特异性和灵敏度，检测靶 DNA 的下限为 0.0001 pg/ul（比 PCR 方法灵敏 10 倍），准确率为 100%。本研究基于 LAMP 技术展示了一种用于检测食品和临床样本中 的潜在快速、经济有效且易于操作的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/667587aad28e/ijerph-18-04401-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/dbad1167c333/ijerph-18-04401-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/44c80ab96953/ijerph-18-04401-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/4c1b729a936f/ijerph-18-04401-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/ec1ca95ab62c/ijerph-18-04401-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/23d0f61d2671/ijerph-18-04401-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/526cdcc02b8e/ijerph-18-04401-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/7fe3f2a6dd0c/ijerph-18-04401-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/e4ca5474cfe0/ijerph-18-04401-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/667587aad28e/ijerph-18-04401-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/dbad1167c333/ijerph-18-04401-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/44c80ab96953/ijerph-18-04401-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/4c1b729a936f/ijerph-18-04401-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/ec1ca95ab62c/ijerph-18-04401-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/23d0f61d2671/ijerph-18-04401-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/526cdcc02b8e/ijerph-18-04401-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/7fe3f2a6dd0c/ijerph-18-04401-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/e4ca5474cfe0/ijerph-18-04401-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3eb/8122632/667587aad28e/ijerph-18-04401-g009.jpg

相似文献

1
Rapid Detection of in Food Using Loop-Mediated Isothermal Amplification (LAMP).利用环介导等温扩增技术（LAMP）快速检测食品中的 。
Int J Environ Res Public Health. 2021 Apr 21;18(9):4401. doi: 10.3390/ijerph18094401.
2
Rapid and simple detection of Clostridium botulinum types A and B by loop-mediated isothermal amplification.采用环介导等温扩增技术快速简便地检测 A 型和 B 型肉毒梭菌。
J Appl Microbiol. 2009 Apr;106(4):1252-9. doi: 10.1111/j.1365-2672.2008.04084.x. Epub 2009 Jan 30.
3
Development and evaluation of a rapid visual loop-mediated isothermal amplification assay for the gene in detection.用于检测 基因的快速目视环介导等温扩增检测法的建立与评价。
PeerJ. 2024 Aug 30;12:e17776. doi: 10.7717/peerj.17776. eCollection 2024.
4
Multiplex PCR for detection of botulinum neurotoxin-producing clostridia in clinical, food, and environmental samples.用于检测临床、食品和环境样本中产生肉毒杆菌神经毒素的梭状芽孢杆菌的多重聚合酶链反应。
Appl Environ Microbiol. 2009 Oct;75(20):6457-61. doi: 10.1128/AEM.00805-09. Epub 2009 Aug 14.
5
Differentiating Botulinum Neurotoxin-Producing Clostridia with a Simple, Multiplex PCR Assay.用简单的多重PCR检测法区分产肉毒杆菌神经毒素的梭状芽孢杆菌
Appl Environ Microbiol. 2017 Aug 31;83(18). doi: 10.1128/AEM.00806-17. Print 2017 Sep 15.
6
Cloth-based hybridization array system for the detection of Clostridium botulinum type A, B, E, and F neurotoxin genes.用于检测A型、B型、E型和F型肉毒梭菌神经毒素基因的基于布的杂交阵列系统。
J Food Prot. 2005 Jul;68(7):1477-83. doi: 10.4315/0362-028x-68.7.1477.
7
Polymerase chain reaction for the rapid identification of Clostridium botulinum type A strains and detection in food samples.用于快速鉴定A型肉毒梭菌菌株及食品样本检测的聚合酶链反应
J Appl Bacteriol. 1993 Sep;75(3):234-9. doi: 10.1111/j.1365-2672.1993.tb02771.x.
8
Development and evaluation of isothermal amplification assay for the rapid and sensitive detection of Clostridium perfringens from chevon.用于快速灵敏检测山羊肉中产气荚膜梭菌的等温扩增检测方法的开发与评估
Anaerobe. 2018 Dec;54:178-187. doi: 10.1016/j.anaerobe.2018.09.005. Epub 2018 Sep 22.
9
Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complex.实时PCR检测无毒非血凝素基因作为携带肉毒杆菌神经毒素（A - G）基因复合体的细菌分离株的快速筛选方法。
J Microbiol Methods. 2007 Dec;71(3):343-6. doi: 10.1016/j.mimet.2007.09.016. Epub 2007 Sep 29.
10
Pentaplexed quantitative real-time PCR assay for the simultaneous detection and quantification of botulinum neurotoxin-producing clostridia in food and clinical samples.五重实时定量 PCR 法检测食品和临床样本中产生肉毒神经毒素的梭状芽胞杆菌
Appl Environ Microbiol. 2010 Jul;76(13):4387-95. doi: 10.1128/AEM.02490-09. Epub 2010 Apr 30.

引用本文的文献

1
Integrating loop-mediated isothermal amplification with lateral flow assay to achieve a highly sensitive method for detecting Genome in raw pork.将环介导等温扩增技术与侧向流动分析法相结合，以实现一种用于检测生猪肉中基因组的高灵敏度方法。
Heliyon. 2024 Aug 28;10(17):e36942. doi: 10.1016/j.heliyon.2024.e36942. eCollection 2024 Sep 15.
2
Health Hazard Associated with the Presence of Bacteria in Food Products.食品中细菌存在所带来的健康危害。
Foods. 2024 Aug 18;13(16):2578. doi: 10.3390/foods13162578.
3
New insights into the roles of fungi and bacteria in the development of medicinal plant.

本文引用的文献

1
Alternative Methods for Testing Botulinum Toxin: Current Status and Future Perspectives.肉毒杆菌毒素检测的替代方法：现状与未来展望
Biomol Ther (Seoul). 2020 Jul 1;28(4):302-310. doi: 10.4062/biomolther.2019.200.
2
and Occurrence in Kazakh Honey Samples.以及哈萨克斯坦蜂蜜样本中的存在情况。
Toxins (Basel). 2019 Aug 13;11(8):472. doi: 10.3390/toxins11080472.
3
Rapid Detection of Botulinum Neurotoxins-A Review.肉毒梭菌神经毒素的快速检测——综述
真菌和细菌在药用植物发育中的作用的新见解。
J Adv Res. 2024 Nov;65:137-152. doi: 10.1016/j.jare.2023.12.007. Epub 2023 Dec 12.
4
Establishment of a Rapid LAMP Assay for and Comparison with the Application of qPCR.用于[具体内容未给出]的快速环介导等温扩增（LAMP）检测方法的建立及其与定量聚合酶链反应（qPCR）应用的比较。
Metabolites. 2023 Jul 12;13(7):841. doi: 10.3390/metabo13070841.
5
LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.基于 LAMP 的即时检测生物传感器用于快速病原体检测。
Biosensors (Basel). 2022 Nov 23;12(12):1068. doi: 10.3390/bios12121068.
6
One-Pot Isothermal LAMP-CRISPR-Based Assay for Klebsiella pneumoniae Detection.一锅等温 LAMP-CRISPR 法检测肺炎克雷伯菌。
Microbiol Spectr. 2022 Aug 31;10(4):e0154522. doi: 10.1128/spectrum.01545-22. Epub 2022 Jul 20.
7
A Review of Isothermal Amplification Methods and Food-Origin Inhibitors against Detecting Food-Borne Pathogens.等温扩增方法及食品源性抑制剂对食源性病原体检测的综述
Foods. 2022 Jan 24;11(3):322. doi: 10.3390/foods11030322.
Toxins (Basel). 2019 Jul 17;11(7):418. doi: 10.3390/toxins11070418.
4
Why Are Botulinum Neurotoxin-Producing Bacteria So Diverse and Botulinum Neurotoxins So Toxic?为什么产肉毒杆菌神经毒素的细菌如此多样，而肉毒神经毒素又如此致命？
Toxins (Basel). 2019 Jan 11;11(1):34. doi: 10.3390/toxins11010034.
5
Foodborne botulism: an evolving public health challenge.食源性肉毒中毒：一个不断演变的公共卫生挑战。
Infect Dis (Lond). 2019 Feb;51(2):97-101. doi: 10.1080/23744235.2018.1524584. Epub 2018 Nov 30.
6
Identification and characterization of Clostridium botulinum strains associated with an infant botulism case in China.鉴定和表征与中国婴儿肉毒中毒病例相关的肉毒梭菌菌株。
Anaerobe. 2019 Feb;55:1-7. doi: 10.1016/j.anaerobe.2018.06.015. Epub 2018 Jul 2.
7
Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies.检测生物威胁因子：从现有诊断方法到开发新型传感器技术。
ACS Sens. 2018 Oct 26;3(10):1894-2024. doi: 10.1021/acssensors.8b00420. Epub 2018 Sep 11.
8
Botulinum Neurotoxin Detection Methods for Public Health Response and Surveillance.用于公共卫生应对和监测的肉毒杆菌神经毒素检测方法
Front Bioeng Biotechnol. 2018 Jun 22;6:80. doi: 10.3389/fbioe.2018.00080. eCollection 2018.
9
Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits.使用四种商用DNA提取试剂盒通过实时PCR检测农场环境样本中的肉毒梭菌III型。
BMC Res Notes. 2018 Jul 4;11(1):441. doi: 10.1186/s13104-018-3549-5.
10
Inactivation of non-proteolytic Clostridium botulinum type E in low-acid foods and phosphate buffer by heat and pressure.热压法灭活低酸性食品和磷酸盐缓冲液中无蛋白水解活性的肉毒梭菌 E 型。
PLoS One. 2018 Jul 3;13(7):e0200102. doi: 10.1371/journal.pone.0200102. eCollection 2018.