小鼠腺病毒1型两个分离株的早期区域4序列及生物学比较

Early region 4 sequence and biological comparison of two isolates of mouse adenovirus type 1.

作者信息

Ball A O, Beard C W, Villegas P, Spindler K R

机构信息

Department of Genetics, University of Georgia, Athens 30602.

出版信息

Virology. 1991 Jan;180(1):257-65. doi: 10.1016/0042-6822(91)90030-f.

DOI:10.1016/0042-6822(91)90030-f

PMID:1845825

Abstract

The DNA sequence of 88-100 map units of mouse adenovirus type 1 (MAV-1) was determined. One translational open reading frame showed 48% sequence similarity to a human adenovirus type 2 early region 4 protein. Based on the protein similarity, genome location, and transcriptional polarity, we concluded that this region of MAV-1 corresponds to early region 4. A 241-bp sequence consisting of 10 imperfect direct repeats with sequence similarity to minisatellite DNA was found in this region. Two virus isolates with different passage histories were examined and were found to have a sequence polymorphism within this region. The two viruses were compared for growth in cell culture and mice and small quantitative differences were observed only in vivo.

摘要

测定了1型小鼠腺病毒（MAV-1）88 - 100个图谱单位的DNA序列。一个翻译开放阅读框与2型人腺病毒早期区域4蛋白显示出48%的序列相似性。基于蛋白质相似性、基因组定位和转录极性，我们得出结论，MAV-1的该区域对应于早期区域4。在该区域发现了一个由10个与小卫星DNA具有序列相似性的不完全直接重复序列组成的241bp序列。检查了两种具有不同传代历史的病毒分离株，发现该区域内存在序列多态性。比较了这两种病毒在细胞培养物和小鼠中的生长情况，仅在体内观察到了微小的定量差异。