Enevold Anders, Lusingu John P, Mmbando Bruno, Alifrangis Michael, Lemnge Martha M, Bygbjerg Ib C, Theander Thor G, Vestergaard Lasse S
Centre for Medical Parasitology, Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
Am J Trop Med Hyg. 2008 May;78(5):714-20.
The prevalence of human red blood cell (RBC) polymorphisms is high in areas of intense Plasmodium falciparum transmission, and individuals carrying these genetic traits are believed to be partially protected against severe malaria. However, it remains uncertain how RBC polymorphisms affect the susceptibility to uncomplicated malaria. We compared the risk of suffering from febrile, uncomplicated malaria between individuals carrying three common RBC polymorphisms (sickle cell trait, alpha(+)-thalassemia, and glucose-6-phosphate-dehydrogenase deficiency) and controls. The study was performed in an area of intense malaria transmission where 202 individuals 0-19 years of age were monitored clinically for a period of 6 months. RBC polymorphisms were assessed with molecular methods, and plasma antibodies to P. falciparum variant surface antigens (anti-VSA IgG) and glutamate-rich protein (anti-GLURP IgG) were measured with flow cytometry and ELISA assays, respectively. Regression analyses showed that alpha(+)-thalassemia was associated with a reduced risk of uncomplicated malaria episodes and that this advantageous effect seemed to be more predominant in children older than 5 years of age, but was independent of levels of antibodies to VSA and GLURP.
在恶性疟原虫传播猖獗的地区，人类红细胞（RBC）多态性的流行率很高，并且携带这些遗传特征的个体被认为对重症疟疾有部分保护作用。然而，红细胞多态性如何影响非重症疟疾的易感性仍不确定。我们比较了携带三种常见红细胞多态性（镰状细胞性状、α（+）-地中海贫血和葡萄糖-6-磷酸脱氢酶缺乏症）的个体与对照组患发热性非重症疟疾的风险。该研究在一个疟疾传播猖獗的地区进行，对202名0至19岁的个体进行了为期6个月的临床监测。采用分子方法评估红细胞多态性，分别用流式细胞术和ELISA检测针对恶性疟原虫变异表面抗原的血浆抗体（抗VSA IgG）和富含谷氨酸蛋白的抗体（抗GLURP IgG）。回归分析表明，α（+）-地中海贫血与非重症疟疾发作风险降低有关，这种有利影响在5岁以上儿童中似乎更为明显，但与抗VSA和抗GLURP抗体水平无关。
