Tábora Nelba, Ferrera Annabelle, Bakkers Judith M J E, Massuger Leon F A G, Melchers Willem J G
Department of Microbiology, Universidad Nacional Autónoma de Honduras (UNAH), Tegucigalpa, Honduras.
Am J Trop Med Hyg. 2008 May;78(5):843-6.
Cervical cancer is believed to have a co-factorial etiology in which high-risk human papillomavirus (HPV) infections are considered an essential factor and other elements play an ancillary role. Besides the importance of specific HPV genotypes, other viral cofactors as viral load may influence the progression likelihood. In this study the relationship between HPV 16 viral load with respect to the grade of cervical disease in Honduran women was investigated. A real-time PCR allowing quantification of both HPV 16 genome and beta-globin gene to normalize the measuring HPV 16 load in cervical cells was used. The data in 87 women with cervical dysplasia or cervical cancer and in 23 women with a negative Pap smear were evaluated. The highest average of HPV 16 viral load was detected in women with High Squamous Intraepithelial Lesions (HSIL). An increasing amount of HPV in higher cervical lesions was found, which could indicate a dose-response association between viral load and precancerous lesion grade.
宫颈癌被认为具有多因素病因，其中高危型人乳头瘤病毒（HPV）感染被视为一个关键因素，而其他因素起辅助作用。除了特定HPV基因型的重要性外，其他病毒辅助因子如病毒载量可能会影响疾病进展的可能性。在本研究中，调查了洪都拉斯女性中HPV 16病毒载量与宫颈疾病分级之间的关系。采用了一种实时PCR技术，该技术能够对HPV 16基因组和β-珠蛋白基因进行定量，以便对宫颈细胞中HPV 16载量的测量进行标准化。对87例患有宫颈发育异常或宫颈癌的女性以及23例巴氏涂片检查阴性的女性的数据进行了评估。在高度鳞状上皮内病变（HSIL）女性中检测到HPV 16病毒载量的平均水平最高。在更高等级的宫颈病变中发现HPV数量增加，这可能表明病毒载量与癌前病变分级之间存在剂量反应关联。
