Medrado G C B, Machado C B, Valerio P, Sanches M D, Goes A M
Medicine School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Biomed Mater. 2006 Sep;1(3):155-61. doi: 10.1088/1748-6041/1/3/010. Epub 2006 Aug 9.
Cartilage tissue has poor capability of self-repair, especially in the case of severe cartilage damage due to trauma or age-related degeneration. Cell-based tissue engineering using scaffolds has provided an option for the repair of defects in adult cartilage tissue. Mesenchymal stem cells (MSC) and chondrocytes are the two major cell sources for cartilage tissue engineering. The present study combined culture conditions of MSC in a chitosan-gelatin matrix in chondrogenic media to evaluate their effects on MSC viability and chondrogenesis for cartilage tissue engineering. MSC were harvested from rabbit bone marrows and cultured in chondrogenic media supplemented, or not, with dexamethasone in a chitosan-gelatin film (C-GF). The association of C-GF and dexamethasone promoted significant increase in cell adhesivity, viability and proliferation when compared to MCS cultured in media without dexamethasone or C-GF. In addition, dexamethasone promoted increase in the collagen concentration of MSC cultures. A reduction of alkaline phosphatase activity after three weeks of culture in chondrogenic media was verified. No influence of the C-GF or of dexamethasone was observed in this matter. Therefore, it is reasonable to suggest that biomaterial-based chitosan-gelatin and chondrogenic media supplemented with dexamethasone may stimulate the proliferation and differentiation of MSC according to the complex environmental conditions. The information presented here should be useful for the development of biomaterials to regulate the chondrogenesis of MSC suitable for cartilage tissue engineering.
软骨组织的自我修复能力较差，尤其是在因创伤或年龄相关退变导致严重软骨损伤的情况下。使用支架的基于细胞的组织工程为修复成人软骨组织缺损提供了一种选择。间充质干细胞（MSC）和软骨细胞是软骨组织工程的两种主要细胞来源。本研究将MSC在壳聚糖-明胶基质中于软骨生成培养基中的培养条件相结合，以评估它们对软骨组织工程中MSC活力和软骨生成的影响。从兔骨髓中获取MSC，并在壳聚糖-明胶膜（C-GF）中于添加或不添加地塞米松的软骨生成培养基中培养。与在无地塞米松或C-GF的培养基中培养的MSC相比，C-GF与地塞米松的联合使用显著提高了细胞黏附性、活力和增殖。此外，地塞米松促进了MSC培养物中胶原蛋白浓度的增加。在软骨生成培养基中培养三周后，碱性磷酸酶活性降低得到了验证。在此方面未观察到C-GF或地塞米松的影响。因此，有理由认为基于生物材料的壳聚糖-明胶和添加地塞米松的软骨生成培养基可能根据复杂的环境条件刺激MSC的增殖和分化。此处提供的信息应有助于开发用于调节适合软骨组织工程的MSC软骨生成的生物材料。
