Rasti Boroojen Fatemeh, Mashayekhan Shohreh, Abbaszadeh Hojjat-Allah
Department of chemical and petroleum engineering, Sharif University of technology, , Tehran, Iran.
Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2019 Winter;18(1):111-124.
In this study, a system of dexamethasone sodium phosphate (DEXP)-loaded chitosan nanoparticles embedded in poly-ε-caprolacton (PCL) and gelatin electrospun nanofiber scaffold was introduced with potential therapeutic application for treatment of the nervous system. Besides anti-inflammatory properties, DEXP act through its glucocorticoid receptors, which are involved in the inhibition of astrocyte proliferation and microglial activation. Bovine serum albumin (BSA) was used to improve the encapsulation efficiency of DEXP within chitosan nanoparticles and to overcome its initial burst release. BSA incorporation within the chitosan nanoparticles increased the encapsulation efficiency of DEXP from 30% to 77%. The comparison between DEXP release profile from PCL/gelatin scaffold with and without chitosan nanoparticles revealed that the system of DEXP-BSA-loaded chitosan nanoparticles embedded in electrospun PCL nanofiber scaffold provided a more controlled release pattern of the loaded drug. The scaffolds properties in terms of structure, hydrophilicity, cell compatibility, mechanical property, and biodegradability were further investigated, which might show its potential application for the repair of spinal cord injury.
在本研究中，引入了一种将负载地塞米松磷酸钠（DEXP）的壳聚糖纳米颗粒嵌入聚ε-己内酯（PCL）和明胶电纺纳米纤维支架的系统，该系统在治疗神经系统疾病方面具有潜在的治疗应用价值。除了具有抗炎特性外，DEXP还通过其糖皮质激素受体发挥作用，这些受体参与抑制星形胶质细胞增殖和小胶质细胞活化。牛血清白蛋白（BSA）用于提高DEXP在壳聚糖纳米颗粒中的包封效率，并克服其初始突释现象。壳聚糖纳米颗粒中加入BSA后，DEXP的包封效率从30%提高到了77%。对含和不含壳聚糖纳米颗粒的PCL/明胶支架中DEXP释放曲线的比较表明，嵌入电纺PCL纳米纤维支架中的负载DEXP-BSA的壳聚糖纳米颗粒系统提供了一种更可控的负载药物释放模式。进一步研究了支架在结构、亲水性、细胞相容性、机械性能和生物降解性方面的特性，这些特性可能显示出其在脊髓损伤修复中的潜在应用价值。
