Cao Xian-Ying, Yin Mei-Zhen, Zhang Li-Na, Li Shi-Pu, Cao Yang
Science and Engineering College of Hainan University, Key Laboratory of National Ministry of Education: Research and Application of Tropical Aquatic and Terrestrial Biological Resources, Haikou 570228, People's Republic of China. cxying
Biomed Mater. 2006 Dec;1(4):L16-9. doi: 10.1088/1748-6041/1/4/L02. Epub 2006 Sep 25.
To establish an experimental model for culturing rabbit osteoblasts in vitro, the osteoblasts were isolated from the calvarial bone of a 15-day old rabbit using a method of culturing the bone pieces in a medium after they had been digested by an enzyme for 15 min. The acquired cells were assayed by cell morphology, alkaline phosphatase activity and production of a mineralized matrix. The results showed that the cells had the morphologic characteristics and some biological behaviours of osteoblasts. Based on the primary isolation of osteoblasts from bone and combining digestion with explants, a novel model for culturing rabbit osteoblasts in vitro was established, which is easy, efficient and effective. This model can be used in many studies of osteogenesis mechanisms and bone replacement materials.
为建立体外培养兔成骨细胞的实验模型，采用酶消化15分钟后将骨块置于培养基中培养的方法，从15日龄兔的颅骨中分离成骨细胞。通过细胞形态、碱性磷酸酶活性和矿化基质生成对获取的细胞进行检测。结果显示，这些细胞具有成骨细胞的形态特征和一些生物学行为。基于从骨中初次分离成骨细胞并结合消化与组织块培养，建立了一种新型的体外培养兔成骨细胞的模型，该模型简便、高效且有效。此模型可用于许多成骨机制和骨替代材料的研究。
