Andrade G, Barbosa-Stancioli E F, Piscitelli Mansur A A, Vasconcelos W L, Mansur H S
Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, PO Box 486, 31270.901, Belo Horizonte, MG, Brazil.
Biomed Mater. 2006 Dec;1(4):221-34. doi: 10.1088/1748-6041/1/4/008. Epub 2006 Dec 4.
The purpose of this study was to develop novel hybrid organic-inorganic materials based on poly(vinyl alcohol) (PVA) polymer chemically crosslinked network to be tested as solid support on bovine herpesvirus immunoassay. Hybrids were synthesized by reacting PVA with three different alkoxysilanes modifying chemical groups: tetraethoxysilane (TEOS), 3-mercaptopropyltrimethoxysilane (MPTMS) and 3-glycidoxypropyltrimethoxysilane (GPTMS). PVA-derived hybrids were also modified by chemically crosslinking with glutaraldehyde (GA) during the synthesis reaction. In order to investigate the structure in the nanometer-scale, PVA-derived hybrids were characterized by using small-angle x-ray scattering synchrotron radiation (SAXS) and x-ray diffraction (XRD). PVA hybrids' chemical functionalities and their interaction with herpesviruses were also characterized by Fourier transform infrared spectroscopy (FTIR). The bioactivity assays were tested through enzyme linked immunosorbent assay (ELISA). SAXS results have indicated nano-ordered disperse domains for PVA hybrids with different x-ray scattering patterns for PVA polymer and PVA-derived hybrids. FTIR spectra have shown major vibration bands associated with organic-inorganic chemical groups present in the PVA, PVA-derived by silane modifier and PVA chemically crosslinked by GA. The immunoassay results have shown that PVA hybrids with chemically functionalized structures regulated to some extent the specific bioimmobilization of herpesvirus onto solid phase. We think that it is due to the overall balance of forces associated with van der Waals interaction, hydrophilic and hydrophobic forces and steric hindrance acting at the surface. PVA and PVA-derived hybrid materials were successfully produced with GA crosslinking in a nanometer-scale network. Also, such a PVA-based material could be advantageously used in immunoassays with enhanced specificity for diagnosis.
本研究的目的是基于化学交联网络的聚乙烯醇（PVA）聚合物开发新型有机-无机杂化材料，以作为牛疱疹病毒免疫测定的固相支持物进行测试。通过使PVA与三种不同的修饰化学基团的烷氧基硅烷反应来合成杂化物：四乙氧基硅烷（TEOS）、3-巯基丙基三甲氧基硅烷（MPTMS）和3-环氧丙氧基丙基三甲氧基硅烷（GPTMS）。在合成反应过程中，通过与戊二醛（GA）化学交联对PVA衍生的杂化物进行了改性。为了研究纳米尺度的结构，使用小角X射线散射同步辐射（SAXS）和X射线衍射（XRD）对PVA衍生的杂化物进行了表征。还通过傅里叶变换红外光谱（FTIR）对PVA杂化物的化学官能团及其与疱疹病毒的相互作用进行了表征。通过酶联免疫吸附测定（ELISA）测试了生物活性测定。SAXS结果表明，PVA聚合物和PVA衍生的杂化物具有不同的X射线散射模式，PVA杂化物具有纳米级有序分散域。FTIR光谱显示了与PVA中存在的有机-无机化学基团、硅烷改性剂衍生的PVA以及GA化学交联的PVA相关的主要振动带。免疫测定结果表明，具有化学官能化结构的PVA杂化物在一定程度上调节了疱疹病毒在固相上的特异性生物固定。我们认为这是由于范德华相互作用、亲水和疏水作用力以及表面作用的空间位阻相关的总作用力平衡所致。通过GA交联在纳米级网络中成功制备了PVA和PVA衍生的杂化材料。此外，这种基于PVA的材料可有利地用于具有增强诊断特异性的免疫测定中。
