Cökeliler D, Caner H, Zemek J, Choukourov A, Biederman H, Mutlu M
Plasma Aided Bioengineering and Biotechnology Research Laboratory, Engineering Faculty, Hacettepe University, 06532, Ankara, Turkey.
Biomed Mater. 2007 Mar;2(1):39-47. doi: 10.1088/1748-6041/2/1/007. Epub 2007 Jan 19.
Prosthetic devices, mainly shunts, are frequently used for temporary or permanent drainage of cerebrospinal fluid. The pathogenesis of shunt infection is a very important problem in modern medicine and generally this is characterized by staphylococcal adhesion to the cerebrospinal fluid shunt surfaces. In this paper, the prevention of the attachment of test microorganism Staphylococcus epidermidis on the cerebrospinal fluid shunt surfaces by 2-hydroxyethylmethacrylate (HEMA) precursor modification in the plasma polymerization system, is reported. Different plasma polymerization conditions (RF discharge power 10-20-30 W, exposure time 5-10-15 min) were employed during the surface modification. The surface chemistry and topology of unmodified and modified shunts was characterized by x-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and atomic force microscopy (AFM). Also, static contact angle measurements were performed to state the change of surface hydrophilicity. All samples were tested in vitro with Staphylococcus epidermidis. A plasma-polymerized HEMA film (PP HEMA) was found to be an alternative simple method to decrease the microorganism attachment and create bacterial anti-fouling surfaces. The attachment of the model microorganism Staphylococcus epidermidis on the shunt surface modified by PP HEMA at 20 W and 15 min was reduced 62.3% if compared to the unmodified control surface of the shunt.
假体装置，主要是分流器，常用于脑脊液的临时或永久性引流。分流感染的发病机制是现代医学中的一个非常重要的问题，一般来说，其特征是葡萄球菌粘附于脑脊液分流器表面。本文报道了在等离子体聚合体系中通过甲基丙烯酸羟乙酯（HEMA）前体改性来防止测试微生物表皮葡萄球菌附着在脑脊液分流器表面的情况。在表面改性过程中采用了不同的等离子体聚合条件（射频放电功率10 - 20 - 30 W，暴露时间5 - 10 - 15分钟）。通过X射线光电子能谱（XPS）、扫描电子显微镜（SEM）和原子力显微镜（AFM）对未改性和改性分流器的表面化学和拓扑结构进行了表征。此外，还进行了静态接触角测量以说明表面亲水性的变化。所有样品均在体外与表皮葡萄球菌进行测试。发现等离子体聚合的HEMA膜（PP HEMA）是一种减少微生物附着并创建细菌防污表面的替代简单方法。与分流器未改性的对照表面相比，在20 W和15分钟条件下用PP HEMA改性的分流器表面上，模型微生物表皮葡萄球菌的附着减少了62.3%。
