Department of Neurosurgery, Clinical Medicine, Tsukuba University, Tsukuba, Ibaraki, Japan.
Acta Biomater. 2010 Jul;6(7):2751-9. doi: 10.1016/j.actbio.2009.12.045. Epub 2010 Jan 4.
Our objective was to develop a bone substitute coated with fibroblast growth factor-2 (FGF-2) that subsequently releases FGF-2. We investigated the use of our system of bone substitutes to induce bone formation. Hydroxyapatite ceramic buttons (HAP-CBs) were coated with FGF-2 by precipitation in supersaturated calcium phosphate solution. HAP-CBs were coated with high or low doses of FGF-2, denoted as FGF-H and FGF-L. The release of FGF-2 from FGF-H and FGF-L was evaluated using its release profile and bioactivity. The efficacy of the subsequent bone formation was quantified using rats with round-shaped bone defects (5mm in diameter) of the right parietal bone. Group 1 was treated only with HAP-CBs, group 2 with HAP-CBs and drops of FGF-2 solution, group 3 with FGF-L and group 4 with FGF-H. To detect the release of FGF-2 in vivo, the expression of bone morphogenic protein-2 (BMP-2) was measured in the defective bone tissue. FGF-2 was released in vitro from FGF-H and FGF-L, and maintained its bioactivity. Rats treated with FGF-L showed better bone formation than rats from the other groups. BMP-2 expression was detected in the defective bone tissues of group 3 at 14 days, which might indicate in vivo FGF-2 release during this period. A specific FGF-2 concentration may be needed for bone formation, and our system can release FGF-2 at adequate concentrations to induce bone formation.
我们的目标是开发一种表面涂覆有成纤维细胞生长因子-2（FGF-2）的骨替代物，使其随后释放 FGF-2。我们研究了使用我们的骨替代物系统来诱导骨形成。通过在过饱和的磷酸钙溶液中沉淀，将纤维蛋白生长因子-2涂覆在羟基磷灰石陶瓷纽扣（HAP-CBs）上。HAP-CBs 用高剂量或低剂量的 FGF-2 涂覆，分别表示为 FGF-H 和 FGF-L。通过释放曲线和生物活性评估 FGF-H 和 FGF-L 中 FGF-2 的释放。使用右侧顶骨上具有圆形骨缺损（直径 5mm）的大鼠来定量评估随后骨形成的功效。第 1 组仅用 HAP-CBs 处理，第 2 组用 HAP-CBs 和 FGF-2 溶液滴处理，第 3 组用 FGF-L 处理，第 4 组用 FGF-H 处理。为了检测 FGF-2 在体内的释放，测量了缺损骨组织中骨形成蛋白-2（BMP-2）的表达。FGF-H 和 FGF-L 在体外释放 FGF-2，并保持其生物活性。用 FGF-L 治疗的大鼠比其他组的大鼠表现出更好的骨形成。第 3 组的缺损骨组织在 14 天检测到 BMP-2 表达，这可能表明在此期间体内释放了 FGF-2。形成骨可能需要特定的 FGF-2 浓度，而我们的系统可以以足够的浓度释放 FGF-2 以诱导骨形成。
