Olivier V, Hivart Ph, Descamps M, Hardouin P
Laboratoire de Recherche sur les Biomatériaux et les Biotechnologies (LR2B, UPRES EA 2603), Université du Littoral Côte-d'Opale, Boulogne-sur-Mer, France.
Biomed Mater. 2007 Sep;2(3):174-80. doi: 10.1088/1748-6041/2/3/002. Epub 2007 Aug 23.
New biomaterials combined with osteogenic cells are now being developed as an alternative to autogeneous bone grafts when the skeletal defect reaches a critical size. Yet, the size issue appears to be a key obstacle in the development of bone tissue engineering. Bioreactors are needed to allow the in vitro expansion of cells inside large bulk materials under appropriate conditions. However, no bioreactor has yet been designed for large-scale 3D structures and custom-made scaffolds. In this study, we evaluate the efficiency of a new bioreactor for the in vitro development of large bone substitutes, ensuring the perfusion of large ceramic scaffolds by the nutritive medium. The survival and proliferation of cells inside the scaffolds after 7 and 28 days in this dynamic culture system and the impact of the direction of the flow circulation are evaluated. The follow-up of glucose consumption, DNA quantification and microscopic evaluation all confirmed cell survival and proliferation for a sample under dynamic culture conditions, whereas static culture leads to the death of cells inside the scaffolds. Two directions of flow perfusion were assayed; the convergent direction leads to enhanced results compared to divergent flow.
当骨骼缺损达到临界大小时，新型生物材料与成骨细胞相结合正被开发出来作为自体骨移植的替代物。然而，尺寸问题似乎是骨组织工程发展的关键障碍。需要生物反应器来使细胞在适当条件下在大块材料内部进行体外扩增。然而，尚未设计出用于大规模三维结构和定制支架的生物反应器。在本研究中，我们评估了一种新型生物反应器在体外培养大型骨替代物方面的效率，确保营养培养基对大型陶瓷支架的灌注。评估了在这种动态培养系统中培养7天和28天后支架内细胞的存活和增殖情况以及流动循环方向的影响。对葡萄糖消耗、DNA定量和显微镜评估的跟踪均证实，在动态培养条件下样品中的细胞存活并增殖，而静态培养则导致支架内细胞死亡。检测了两种流动灌注方向；与发散流相比，汇聚方向产生了更好的结果。
