Kallajoki M, Hyypiä T, Halonen P, Orvell C, Rima B K, Kalimo H
Department of Pathology, University of Turku, Finland.
Hum Pathol. 1991 Jan;22(1):29-32. doi: 10.1016/0046-8177(91)90057-v.
Inclusion body myositis (IBM) is a distinct type of muscle disease. The characteristic electron microscopic findings, intranuclear or intracytoplasmic inclusions composed of microtubular filaments, morphologically resemble paramyxovirus nucleocapsids. These findings and the reported immunoreactivity of the inclusions with mumps virus antibodies have suggested that inclusion body myositis is a chronic virus infection. We analyzed skeletal muscle specimens from three patients with characteristic light microscopic features and electron microscopically verified inclusions of IBM by immunocytochemistry using antibodies raised against members of the paramyxovirus group, and by in situ hybridization with a cRNA probe representing the mumps virus nucleocapsid gene. The specificity of the reactions was demonstrated with infected and uninfected cultured cells. No immunocytochemical staining or hybridization signal was observed in biopsy specimens from IBM patients. These findings speak against a paramyxovirus etiology of IBM.
包涵体肌炎（IBM）是一种独特类型的肌肉疾病。其特征性的电子显微镜检查结果显示，由微管丝组成的核内或胞质内包涵体在形态上类似于副粘病毒核衣壳。这些发现以及所报道的包涵体与腮腺炎病毒抗体的免疫反应性表明，包涵体肌炎是一种慢性病毒感染。我们通过使用针对副粘病毒组成员产生的抗体进行免疫细胞化学分析，以及用代表腮腺炎病毒核衣壳基因的cRNA探针进行原位杂交，对三名具有特征性光学显微镜特征且经电子显微镜证实有IBM包涵体的患者的骨骼肌标本进行了分析。通过感染和未感染的培养细胞证实了反应的特异性。在IBM患者的活检标本中未观察到免疫细胞化学染色或杂交信号。这些发现不支持IBM由副粘病毒引起的病因学观点。
