Nishino H, Engel A G, Rima B K
Department of Neurology, Mayo Clinic, Rochester, MN 55905.
Ann Neurol. 1989 Mar;25(3):260-4. doi: 10.1002/ana.410250309.
The resemblance of the filamentous inclusions in inclusion body myositis (IBM) to mumps virus nucleoproteins and the report of immunoreactivity of the inclusions for mumps virus antigens have implicated the mumps virus in the etiology of IBM. We tested the mumps virus hypothesis by in-situ hybridization with a cDNA probe specific for the mumps virus nucleocapsid gene, and immunocytochemically with antibodies against "soluble" and "viral" mumps antigens. The tests were performed on muscle specimens (IBM, 20; acid maltase deficiency, 4; chloroquine myopathy, 2; nonweak control subjects, 5) and mumps virus-infected and uninfected HEp-2 cells. The in-situ hybridization study showed a strong specific signal in the infected HEp-2 cells but no specific signal in IBM, other myopathies, or nonweak control subjects. The immunocytochemical study showed specific binding of the antimumps antibodies to the infected HEp-2 cells but demonstrated only nonspecific binding of these antibodies around rimmed vacuoles in IBM, acid maltase deficiency, and chloroquine myopathy. These studies cast doubt on the mumps hypothesis of IBM.
包涵体肌炎（IBM）中丝状包涵体与腮腺炎病毒核蛋白的相似性，以及关于这些包涵体对腮腺炎病毒抗原具有免疫反应性的报道，提示腮腺炎病毒与IBM的病因有关。我们通过用针对腮腺炎病毒核衣壳基因的cDNA探针进行原位杂交，以及用抗“可溶性”和“病毒”腮腺炎抗原的抗体进行免疫细胞化学检测，来检验腮腺炎病毒假说。检测在肌肉标本（IBM患者20例；酸性麦芽糖酶缺乏症患者4例；氯喹肌病患者2例；非肌无力对照受试者5例）以及腮腺炎病毒感染和未感染的HEp-2细胞上进行。原位杂交研究显示，在感染的HEp-2细胞中有强烈的特异性信号，但在IBM、其他肌病或非肌无力对照受试者中未发现特异性信号。免疫细胞化学研究显示，抗腮腺炎抗体与感染的HEp-2细胞有特异性结合，但在IBM、酸性麦芽糖酶缺乏症和氯喹肌病中，仅在镶边空泡周围显示这些抗体的非特异性结合。这些研究对IBM的腮腺炎假说提出了质疑。
