Koppisch Andrew T, Dhungana Suraj, Hill Karen K, Boukhalfa Hakim, Heine Henry S, Colip Leslie A, Romero Raymond B, Shou Yulin, Ticknor Lawrence O, Marrone Babetta L, Hersman Larry E, Iyer Srinivas, Ruggiero Christy E
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Biometals. 2008 Oct;21(5):581-9. doi: 10.1007/s10534-008-9144-9. Epub 2008 May 6.
Petrobactin is the primary siderophore synthesized by Bacillus anthracis str Sterne and is required for virulence of this organism in a mouse model. The siderophore's biosynthetic machinery was recently defined and gene homologues of this operon exist in several other Bacillus strains known to be mammalian pathogens, but are absent in several known to be harmless such as B. subtilis and B. lichenformis. Thus, a common hypothesis regarding siderophore production in Bacillus species is that petrobactin production is exclusive to pathogenic isolates. In order to test this hypothesis, siderophores produced by 106 strains of an in-house library of the Bacillus cereus sensu lato group were isolated and identified using a MALDI-TOF-MS assay. Strains were selected from a previously defined phylogenetic tree of this group in order to include both known pathogens and innocuous strains. Petrobactin is produced by pathogenic strains and innocuous isolates alike, and thus is not itself indicative of virulence.
铁载体是炭疽芽孢杆菌斯特恩菌株合成的主要铁载体，在小鼠模型中，该菌株的致病性需要铁载体。最近确定了这种铁载体的生物合成机制，并且在其他几种已知为哺乳动物病原体的芽孢杆菌菌株中存在该操纵子的基因同源物，但在几种已知无害的菌株中不存在，如枯草芽孢杆菌和地衣芽孢杆菌。因此，关于芽孢杆菌属中铁载体产生的一个常见假说是，铁载体的产生是致病性分离株所特有的。为了验证这一假说，使用基质辅助激光解吸电离飞行时间质谱分析法，从蜡样芽孢杆菌复合群内部文库的106个菌株中分离并鉴定了铁载体。从该复合群先前定义的系统发育树中选择菌株，以包括已知的病原体和无害菌株。致病性菌株和无害分离株都能产生铁载体，因此铁载体本身并不表明具有致病性。
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