Koppisch Andrew T, Browder Cindy C, Moe Amanda L, Shelley Jacob T, Kinkel Blaine A, Hersman Larry E, Iyer Srinivas, Ruggiero Christy E
Bioscience Division, B-4, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Biometals. 2005 Dec;18(6):577-85. doi: 10.1007/s10534-005-1782-6.
The siderophores of Bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. Bacillus anthracis str. Sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of XAD-2 resin, reverse-phase FPLC, and size exclusion chromatography. A combination of 1H and 13C NMR spectroscopy, UV spectroscopy and ESI-MS/MS fragmentation were used to identify the primary siderophore as petrobactin, a catecholate species containing unusual 3,4-dihydroxybenzoate moieties, previously only identified in extracts of Marinobacter hydrocarbonoclasticus. A secondary siderophore was observed and structural analysis of this species is consistent with that reported for bacillibactin, a siderophore observed in many species of bacilli. This is the first structural characterization of a siderophore from B. anthracis, as well as the first characterization of a 3,4-DHB containing catecholate in a pathogen.
炭疽芽孢杆菌的铁载体对该病原体的增殖至关重要，可能对其毒力也必不可少。将炭疽芽孢杆菌斯特恩菌株细胞在无铁培养基中培养，并用XAD - 2树脂、反相快速蛋白质液相色谱法和尺寸排阻色谱法相结合的方法分离和纯化所产生的铁载体。使用1H和13C核磁共振光谱、紫外光谱和电喷雾串联质谱裂解相结合的方法，将主要铁载体鉴定为petrobactin，这是一种含有不寻常的3,4 - 二羟基苯甲酸部分的儿茶酚酸盐类物质，此前仅在嗜油海洋杆菌提取物中鉴定到。观察到一种次要铁载体，对该物质的结构分析与许多芽孢杆菌物种中观察到的铁载体杆菌铁载体的报道一致。这是炭疽芽孢杆菌铁载体的首次结构表征，也是病原体中含3,4 - DHB儿茶酚酸盐的首次表征。
