辛伐他汀与烟酸对酒精处理的Wistar大鼠给药效果的比较。

Comparison of simvastatin and nicotinic acid administration in alcohol-treated Wistar rats.

作者信息

Kolovou Genovefa D, Salpea Klelia D, Mihas Constantinos, Malakos Ioannis, Kafaltis Nikolaos, Bilianou Helen G, Adamopoulou Evdokia N, Mykoniatis Michael, Cokkinos Dennis V

机构信息

1st Cardiology Department, Onassis Cardiac Surgery Centre, Athens, Greece.

出版信息

Hellenic J Cardiol. 2008 Mar-Apr;49(2):79-85.

PMID:18459464

Abstract

INTRODUCTION

Previous studies of ours have shown that simvastatin (S) and nicotinic acid (NA) lower the alcohol (Alc)-induced increase of triglycerides. The aim of this study was to evaluate which drug is more effective and safe in decreasing Alc-induced hypertriglyceridaemia in Wistar rats.

METHODS

Male Wistar rats were randomised into 6 groups, which were fed with: (1) olive oil (Oil group, n=10); (2) Oil + Alc, (Alc group, n=10); (3) S solution in Oil (65 microg/100g body weight), (S group, n=10); (4) NA solution in Oil (8.5 mg/100g body weight), (NA group, n=8); (5) S solution in Oil + AIc (S+Alc group, n=10); and (6) NA solution in Oil + Alc (NA+Alc group, n=9). Another 13 male Wistar rats were fed only a standard laboratory diet (control group). After 8 weeks, blood samples were drawn and the livers were removed. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AP), total cholesterol (TC) and triglycerides (TG) were measured. Liver histopathology was also assessed.

RESULTS

Liver histopathology was similar in all groups and within the normal range. The TG plasma concentration in the Alc group was higher than in the control rats (p < 0.001) or any other groups (Oil, p < 0.001, or S, p < 0.001, or NA, p = 0.003). The Oil, S+Alc, NA+Alc and control groups had similar TG levels, but these were significantly lower compared to the Alc group (p < 0.001). AST plasma concentration was higher in the Alc group compared to controls (p < 0.001), Oil (p < 0.001), S (p < 0.001) and NA (p < 0.001) groups, while the AST concentration in the S+Alc and Na+Alc groups was lower than in the Alc group (p = 0.042, p < 0.001, respectively).

CONCLUSIONS

NA and S, two drugs of different classes, seem to decrease Alc-induced secondary hypertriglyceridaemia to the same extent. Moreover, NA displays a better alleviation of Alc-induced AST raises compared to S, although it enhances small increases in AP and ALT levels.

摘要

引言

我们之前的研究表明，辛伐他汀（S）和烟酸（NA）可降低酒精（Alc）诱导的甘油三酯升高。本研究的目的是评估哪种药物在降低Wistar大鼠Alc诱导的高甘油三酯血症方面更有效且更安全。

方法

将雄性Wistar大鼠随机分为6组，分别给予：（1）橄榄油（油组，n = 10）；（2）橄榄油+酒精（酒精组，n = 10）；（3）油中的S溶液（65微克/100克体重）（S组，n = 10）；（4）油中的NA溶液（8.5毫克/100克体重）（NA组，n = 8）；（5）油中的S溶液+酒精（S +酒精组，n = 10）；以及（6）油中的NA溶液+酒精（NA +酒精组，n = 9）。另外13只雄性Wistar大鼠仅喂食标准实验室饮食（对照组）。8周后，采集血样并取出肝脏。测量丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（AP）、总胆固醇（TC）和甘油三酯（TG）。还评估了肝脏组织病理学。

结果

所有组的肝脏组织病理学相似且在正常范围内。酒精组的TG血浆浓度高于对照大鼠（p < 0.001）或任何其他组（油组，p < 0.001；S组，p < 0.001；NA组，p = 0.003）。油组、S +酒精组、NA +酒精组和对照组的TG水平相似，但与酒精组相比显著更低（p < 0.001）。酒精组的AST血浆浓度高于对照组（p < 0.001）、油组（p < 0.001）、S组（p < 0.001）和NA组（p < 0.001），而S +酒精组和NA +酒精组的AST浓度低于酒精组（分别为p = 0.042，p < 0.001）。