Ghorbel Imen, Elwej Awatef, Jamoussi Kamel, Boudawara Tahia, Kamoun Naziha Grati, Zeghal Najiba
Animal Physiology Laboratory, Sfax Faculty of Sciences, University of Sfax, 3000 Sfax, BP 1171, Tunisia.
Food Funct. 2015 Apr;6(4):1126-35. doi: 10.1039/c4fo01128g.
Extra virgin olive oil has been shown to be effective against oxidative stress associated diseases. In addition to the high quantities of oleic acid, it is rich in phenolic compounds. We investigated the protective efficacy of extra virgin olive oil (EVOO) against the hepatotoxicity induced by both aluminum and acrylamide. Animals were divided into four groups containing six rats each: group 1, serving as controls, received distilled water; group 2 received drinking water containing aluminum chloride (50 mg kg(-1) body weight) and acrylamide (20 mg kg(-1) body weight) by gavage; group 3 received both aluminum and acrylamide in the same ways as well as EVOO (300 μl) by gavage; group 4 received only EVOO by gavage for 3 weeks. The rats exposed to both aluminum and acrylamide exhibited oxidative stress observed by an increase in MDA, AOPP and a decrease in GSH, NPSH and vitamin C levels. The activities of CAT and GPx were decreased, while SOD activity was increased. The liver metallothioneins, such as MT1 and MT2 genes expression, were also increased. EVOO supplementation improved all the parameters mentioned above. The plasma transaminases (AST and ALT), LDH activities, glucose and albumin levels, TC, LDL-C levels, TC/HDL-C and LDL-C/HDL-C ratios were increased, while high density lipoprotein-cholesterol (HDL-C) and TG decreased. The co-administration of EVOO to acrylamide and aluminum treated rats restored their hepatic markers to near-normal values. Liver histological studies confirmed the biochemical parameters and the beneficial role of EVOO. These results suggest that extra virgin olive oil, when added to the diet, may have a beneficial role in decreasing the liver damage induced by both aluminum and acrylamide.
特级初榨橄榄油已被证明对与氧化应激相关的疾病有效。除了含有大量油酸外，它还富含酚类化合物。我们研究了特级初榨橄榄油（EVOO）对铝和丙烯酰胺诱导的肝毒性的保护作用。将动物分为四组，每组六只大鼠：第1组作为对照组，给予蒸馏水；第2组通过灌胃给予含氯化铝（50 mg kg(-1)体重）和丙烯酰胺（20 mg kg(-1)体重）的饮用水；第3组以与第2组相同的方式给予铝和丙烯酰胺，同时通过灌胃给予EVOO（300 μl）；第4组仅通过灌胃给予EVOO，持续3周。暴露于铝和丙烯酰胺的大鼠表现出氧化应激，表现为丙二醛（MDA）、晚期氧化蛋白产物（AOPP）增加，谷胱甘肽（GSH）、非蛋白巯基（NPSH）和维生素C水平降低。过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性降低，而超氧化物歧化酶（SOD）活性增加。肝脏金属硫蛋白，如MT1和MT2基因的表达也增加。补充EVOO改善上述所有参数。血浆转氨酶（AST和ALT）、乳酸脱氢酶（LDH）活性、血糖和白蛋白水平、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）水平、TC/高密度脂蛋白胆固醇（HDL-C）和LDL-C/HDL-C比值升高，而高密度脂蛋白胆固醇（HDL-C）和甘油三酯（TG）降低。将EVOO与丙烯酰胺和铝处理的大鼠共同给药可使它们的肝脏标志物恢复到接近正常的值。肝脏组织学研究证实了生化参数以及EVOO的有益作用。这些结果表明，特级初榨橄榄油添加到饮食中可能对减少铝和丙烯酰胺诱导的肝脏损伤具有有益作用。
