Aslan Kadir, Previte Michael J R, Zhang Yongxia, Gallagher Theresa, Baillie Les, Geddes Chris D
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
Anal Chem. 2008 Jun 1;80(11):4125-32. doi: 10.1021/ac800519r. Epub 2008 May 7.
The use of a combination of low-cost technologies to both extract and detect anthrax DNA from spores and vegetative cells in two steps within 1 min is described. In a cavity, microwave energy is highly focused using thin-film aluminum "bow-tie" structures, to extract DNA from whole spores within 20 s. The detection of the released DNA, from less than 1000 vegetative cells, without additional preprocessing steps is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. The new platform technology presented here is a highly attractive alternative method for DNA extraction and the fast detection of gram-positive bacteria and potentially other pathogenic species and cells as well.
本文描述了一种使用低成本技术组合的方法，该方法能够在1分钟内分两步从孢子和营养细胞中提取并检测炭疽DNA。在一个腔体内，利用薄膜铝“领结”结构将微波能量高度聚焦，从而在20秒内从完整孢子中提取DNA。通过采用微波加速金属增强荧光技术，在另外30秒内即可完成对少于1000个营养细胞释放的DNA的检测，无需额外的预处理步骤。这里展示的新平台技术是一种极具吸引力的替代方法，可用于DNA提取以及革兰氏阳性菌和其他潜在致病物种及细胞的快速检测。
