Ozaki K S, Marques G M, Nogueira E, Feitoza R Q, Cenedeze M A, Franco M F, Mazzali M, Soares M P, Pacheco-Silva A, Câmara N O S
Nephrology Division, Universidade Federal de Säo Paulo, Brazil.
Clin Transplant. 2008 Sep-Oct;22(5):609-16. doi: 10.1111/j.1399-0012.2008.00832.x. Epub 2008 May 4.
Heme oxygenase-1 (HO-1) has a microsatellite polymorphism based on the number of guanosine-thymidine nucleotide repeats (GT) repeats that regulates expression levels and could have an impact on organ survival post-injury. We correlated HO-1 polymorphism with renal graft function. The HO-1 gene was sequenced (N = 181), and the allelic repeats were divided into subclasses: short repeats (S) (<27 repeats) and long repeats (L) (>/=27 repeats). A total of 47.5% of the donors carried the S allele. The allograft function was statistically improved six months, two and three yr after transplantation in patients receiving kidneys from donors with an S allele. For the recipients carrying the S allele (50.3%), the allograft function was also better throughout the follow-up, but reached statistical significance only three yr after transplantation (p = 0.04). Considering only those patients who had chronic allograft nephropathy (CAN; 74 of 181), allograft function was also better in donors and in recipients carrying the S allele, two and three yr after transplantation (p = 0.03). Recipients of kidney transplantation from donors carrying the S allele presented better function even in the presence of CAN.
血红素加氧酶-1（HO-1）具有基于鸟苷-胸腺嘧啶核苷酸重复序列（GT）重复次数的微卫星多态性，该多态性可调节表达水平，并可能对损伤后器官存活产生影响。我们将HO-1多态性与肾移植功能进行了关联研究。对HO-1基因进行了测序（N = 181），并将等位基因重复序列分为亚类：短重复序列（S）（<27次重复）和长重复序列（L）（≥27次重复）。共有47.5%的供者携带S等位基因。在接受携带S等位基因供者肾脏的患者中，移植后6个月、2年和3年时，同种异体移植功能在统计学上得到改善。对于携带S等位基因的受者（50.3%），在整个随访期间同种异体移植功能也更好，但仅在移植后3年达到统计学显著性（p = 0.04）。仅考虑那些患有慢性同种异体移植肾病（CAN；181例中的74例）的患者，在移植后2年和3年时，携带S等位基因的供者和受者的同种异体移植功能也更好（p = 0.03）。即使存在CAN，接受携带S等位基因供者肾脏移植的受者仍表现出更好的功能。
