对位于大肠杆菌K-12的IS3元件α3β3和β5α5内的倒位末端进行核苷酸序列分析。

Nucleotide sequence analysis of the inversion termini located within IS3 elements alpha 3 beta 3 and beta 5 alpha 5 of Escherichia coli K-12.

作者信息

Ajdic D, Jovanovic G, Glisin V, Hejna J, Savic D J

机构信息

Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.

出版信息

J Bacteriol. 1991 Jan;173(2):906-9. doi: 10.1128/jb.173.2.906-909.1991.

DOI:10.1128/jb.173.2.906-909.1991

PMID:1846154

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207089/

Abstract

This paper presents the first detailed structural analysis of termini of an inversion mediated by recombination between Escherichia coli native IS elements. The complete nucleotide sequence of the inversion termini in the lactose region of Escherichia coli K-12 confirms our previous suggestion that the inversion occurred by homologous recombination between alpha 3 beta 3 and beta 5 alpha 5 IS3 elements (D. J. Savic, J. Bacteriol. 140:311-319, 1979; D. J. Savic, S. Romac, and S. D. Ehrlich, J. Bacteriol. 155:943-946, 1983). The data show a slight structural divergence of alpha 3 beta 3 and beta 5 alpha 5 elements, but they do not reveal new sequences within recombined IS3 elements that could influence the expression of nearby genes.

摘要

本文首次详细分析了由大肠杆菌天然插入序列（IS）之间的重组介导的倒位末端结构。大肠杆菌K-12乳糖区域倒位末端的完整核苷酸序列证实了我们之前的推测，即该倒位是由α3β3和β5α5 IS3元件之间的同源重组发生的（D. J. Savic，《细菌学杂志》140:311 - 319，1979；D. J. Savic，S. Romac，和S. D. Ehrlich，《细菌学杂志》155:943 - 946，1983）。数据显示α3β3和β5α5元件存在轻微的结构差异，但未揭示重组IS3元件内可能影响附近基因表达的新序列。