Schwartz E, Kröger M, Rak B
Institut für Biologie III, Universität Freiburg, FRG.
Nucleic Acids Res. 1988 Jul 25;16(14B):6789-802. doi: 10.1093/nar/16.14.6789.
Recently we identified the new insertion (IS) sequence IS150 in various strains of Escherichia coli K-12. We have screened other strains of E. coli and Salmonella typhimurium for the presence of homologous sequences. The strains of E. coli K-12 and W tested contain one or more copies of homology to IS150. We have also determined the complete nucleotide sequence of a copy of IS150 inserted into IS1. Comparison of nucleotide and deduced amino acid sequences of IS150, IS2, IS3, IS51, IS600 and IS629 reveals significant homologies suggesting that these elements are members of a family of phylogenetically related insertion sequences.
最近，我们在多种大肠杆菌K-12菌株中鉴定出了新的插入序列（IS）IS150。我们筛选了其他大肠杆菌菌株和鼠伤寒沙门氏菌，以检测是否存在同源序列。所测试的大肠杆菌K-12和W菌株含有一个或多个与IS150同源的拷贝。我们还确定了插入到IS1中的一个IS150拷贝的完整核苷酸序列。对IS150、IS2、IS3、IS51、IS600和IS629的核苷酸和推导氨基酸序列进行比较，发现了显著的同源性，这表明这些元件是一个系统发育相关插入序列家族的成员。
