Dejaegher Bieke, Mangelings Debby, Vander Heyden Yvan
Department of Analytical Chemistry and Pharmaceutical Technology, Pharmaceutical Institute, Vrije Universiteit Brussel - VUB, Brussels, Belgium.
J Sep Sci. 2008 May;31(9):1438-48. doi: 10.1002/jssc.200700680.
In this review, method development for hydrophilic interaction LC (HILIC) is highlighted. HILIC is a chromatographic technique that uses aqueous-organic solvent mobile phases with a high organic-solvent fraction, and a hydrophilic stationary phase. It is mainly applied for the separation of polar and hydrophilic compounds. Method development, in general, can be done uni- or multivariately. In the univariate approach, the factors that are expected to potentially affect the separation of the compounds will be examined sequentially and one-at-a-time. All HILIC methods found in the literature were developed in this way. For these methods, the analytes, the considered factors, the selected responses, and the finally chosen experimental conditions are discussed in this review. Where examined, the method validation and the comparison with other analytical assay methods is also described. For the multivariate method-development approach, which is based on the use of experimental designs, only a possible strategy is presented, because of the lack of relevant publications in the literature.
本综述重点介绍了亲水作用液相色谱（HILIC）的方法开发。HILIC是一种色谱技术，它使用具有高有机溶剂比例的水-有机溶剂流动相和亲水性固定相。它主要用于分离极性和亲水性化合物。一般来说，方法开发可以单变量或多变量进行。在单变量方法中，预计可能影响化合物分离的因素将依次逐一进行考察。文献中发现的所有HILIC方法都是通过这种方式开发的。对于这些方法，本综述讨论了分析物、考虑的因素、选择的响应以及最终选择的实验条件。在进行考察的情况下，还描述了方法验证以及与其他分析方法的比较。对于基于实验设计的多变量方法开发方法，由于文献中缺乏相关出版物，仅提出了一种可能的策略。
