Department of Analytical Chemistry and Pharmaceutical Technology, Pharmaceutical Institute, Vrije Universiteit Brussel, Brussels, Belgium.
J Sep Sci. 2010 Mar;33(6-7):698-715. doi: 10.1002/jssc.200900742.
In this review, hydrophilic interaction liquid chromatographic (HILIC) applications for pharmaceutical analysis are discussed. The HILIC technique uses an aqueous/organic modifier mobile phase with a high organic modifier fraction and a hydrophilic stationary phase (SP). In general, the SPs are silica or polymer based. The silica-based columns are most often applied and are divided into bare-silica particle, chemically bonded silica particle, and monolithic phases. The bare-silica columns are most frequently used. The much less applied polymer-based columns are also divided into particle and monolithic phases. In this review, the applications are grouped and discussed according to the SP type used. Whenever possible, the considered HILIC application is compared with other HILIC methods, e.g. on other SPs, and/or with other separation modes, for that application. The advantages and drawbacks of HILIC are also discussed, as well as the method validation issues, when executed.
在这篇综述中，讨论了亲水相互作用液相色谱（HILIC）在药物分析中的应用。HILIC 技术使用含有高有机溶剂比例的水/有机溶剂流动相和亲水固定相（SP）。通常，SP 是基于硅胶或聚合物的。硅胶柱最常被应用，分为裸硅胶颗粒、化学键合硅胶颗粒和整体相。裸硅胶柱最常用。应用较少的聚合物柱也分为颗粒和整体相。在这篇综述中，根据所用的 SP 类型对应用进行了分组和讨论。只要有可能，就会将所考虑的 HILIC 应用与其他 HILIC 方法进行比较，例如在其他 SP 上，以及/或与该应用的其他分离模式进行比较。还讨论了 HILIC 的优缺点，以及在执行时的方法验证问题。
