Blumenkrantz N, Asboe-Hansen G
Acta Endocrinol (Copenh). 1976 Nov;83(3):665-72. doi: 10.1530/acta.0.0830665.
The effect of increasing doses of hydrocortisone acetate, hydrocortisone phosphoric acid complex, and hydrocortisone sodium succinate on collagen biosynthesis was assayed in two different systems. I. Explants of chicken embryo tibiae showed decreased biosynthesis of [14C]hydroxyproline and total and glycosylated [14C]hydroxylysine under the influence of high doses of hydrocortisone. With the lower doses of hydrocortisone acetate, no effect was noticed. The total uptake of the precursor amino acids followed patterns similar to those of collagen biosynthesis. II. Hydroxylation of [14C]proline labelled protocollagen by protocollagen proline hydroxylase was inhibited by high doses of hydrocortisone acetate, hydrocortisone phosphoric acid complex, and hydrocortisone sodium succinate. III. A decreased diffusion of collagen to the medium with increasing doses of hydrocortisone acetate, hydrocortisone phosphoric acid, and hydrocortisone sodium succinate was noticed. IV. No further hydroxylation of new-synthesized collagen was obtained under the influence of hydrocortisone phosphoric acid and hydrocortisone sodium succinate, when the undialyzable material was used as a substrate for protocollagen proline hydroxylase.
在两个不同系统中测定了递增剂量的醋酸氢化可的松、氢化可的松磷酸复合物和氢化可的松琥珀酸钠对胶原蛋白生物合成的影响。一、鸡胚胫骨外植体显示，在高剂量氢化可的松的影响下，[14C]羟脯氨酸以及总[14C]羟赖氨酸和糖基化[14C]羟赖氨酸的生物合成减少。使用较低剂量的醋酸氢化可的松时，未观察到影响。前体氨基酸的总摄取模式与胶原蛋白生物合成的模式相似。二、高剂量的醋酸氢化可的松、氢化可的松磷酸复合物和氢化可的松琥珀酸钠抑制了原胶原蛋白脯氨酸羟化酶对[14C]脯氨酸标记的原胶原蛋白的羟化作用。三、随着醋酸氢化可的松、氢化可的松磷酸和氢化可的松琥珀酸钠剂量的增加，观察到胶原蛋白向培养基中的扩散减少。四、当不可透析物质用作原胶原蛋白脯氨酸羟化酶的底物时，在氢化可的松磷酸和氢化可的松琥珀酸钠的影响下，新合成的胶原蛋白未进一步羟化。
