通过光学透射和发射断层扫描对未切片标本进行光学清除以实现三维成像。
Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography.
作者信息
Oldham Mark, Sakhalkar Harshad, Oliver Tim, Allan Johnson G, Dewhirst Mark
机构信息
Duke University Medical Center, Department of Radiation Oncology, Durham, North Carolina 27710, USA.
出版信息
J Biomed Opt. 2008 Mar-Apr;13(2):021113. doi: 10.1117/1.2907968.
Abstract
Optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are new techniques that enable unprecedented high-resolution 3-D multimodal imaging of tissue structure and function. Applications include imaging macroscopic gene expression and microvasculature structure in unsectioned biological specimens up to 8 cm(3). A key requisite for these imaging techniques is effective sample preparation including optical clearing, which enables light transport through the sample while preserving the signal (either light absorbing stain or fluorescent proteins) in representative form. We review recent developments in optical-CT and optical-ECT, and compatible "fluorescence-friendly" optical clearing protocols.
摘要
光学计算机断层扫描（光学CT）和光发射计算机断层扫描（光学ECT）是新技术，能够对组织结构和功能进行前所未有的高分辨率三维多模态成像。其应用包括对最大达8立方厘米的未切片生物标本中的宏观基因表达和微血管结构进行成像。这些成像技术的一个关键要求是有效的样品制备，包括光学透明化处理，这能使光透过样品，同时以代表性形式保留信号（吸光染料或荧光蛋白）。我们综述了光学CT和光学ECT以及兼容的“荧光友好型”光学透明化方案的最新进展。
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本文引用的文献
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