Mir Drikvand Reza, Sohrabi Seyyed Mohsen, Samiei Kamran
Department of Agronomy and Plant Breeding, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
Young Researchers and Elite Club, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
3 Biotech. 2019 Mar;9(3):104. doi: 10.1007/s13205-019-1617-8. Epub 2019 Feb 23.
Six full-length gene and cDNA sequences of defensin were identified from L. plant. The identified genes and cDNAs were different in length and their coding sequences contained Knot1 functional domain. Phylogenetic analysis classified the identified defensins into two subfamilies. All defensin genes contained only one intron and had extracellular signal peptides. Secondary structures of identified defensins were completely composed of alpha helix and beta strand. Presence of conserved Cys amino acids and disulfide bridges, interaction with defense and signaling proteins and antimicrobial activity were other common features of these peptides. The identified defensins displayed differential expression pattern in the various tissues. The highest expression level of defensins was observed in seed, pod, and root tissues. Defensin 4 was significantly expressed in all examined tissues, whereas the other defensins were only expressed in some tissues. Also, in the fungal and wounding treatments, lentil defensins showed different expression pattern. Defensin 1 was up-regulated in both fungal and wounding treatments. Defensin 4 showed decreased expression level in both fungal and wounding treatments. Defensins 2 and 6 were up-regulated in wounding and fungal treatments, respectively. In this study, for the first time, six defensin genes were isolated and characterized from lentil. Our results highlighted the role of defensins in lentil plant that can be used for future studies.
从扁豆中鉴定出6个防御素的全长基因和cDNA序列。所鉴定的基因和cDNA长度不同，其编码序列包含Knot1功能域。系统发育分析将所鉴定的防御素分为两个亚家族。所有防御素基因仅包含一个内含子，并具有细胞外信号肽。所鉴定防御素的二级结构完全由α螺旋和β链组成。保守半胱氨酸氨基酸和二硫键的存在、与防御和信号蛋白的相互作用以及抗菌活性是这些肽的其他共同特征。所鉴定的防御素在不同组织中表现出差异表达模式。在种子、豆荚和根组织中观察到防御素的最高表达水平。防御素4在所有检测组织中均显著表达，而其他防御素仅在某些组织中表达。此外，在真菌和创伤处理中，扁豆防御素表现出不同的表达模式。防御素1在真菌和创伤处理中均上调。防御素4在真菌和创伤处理中均表现出表达水平下降。防御素2和6分别在创伤和真菌处理中上调。在本研究中，首次从扁豆中分离并鉴定了6个防御素基因。我们的结果突出了防御素在扁豆植物中的作用，可用于未来的研究。
