Arner P
Acta Med Scand. 1976;200(3):179-86. doi: 10.1111/j.0954-6820.1976.tb08217.x.
Human subcutaneous adipose tissue has been incubated in vitro in the presence and absence of isoprenaline (ISNA). The tissue concentration of cyclic AMP (cAMP) and the release of glycerol into the incubation medium were measured after various incubation periods. In the presence of ISNA (6 X 10(-5) mol/l), the tissue concentration of cAMP reached a peak after around 10 min and then declined to a level significantly lower than that at the start of the incubation. In contrast, the ISNA-induced rate of lipolysis was a linear function of the incubation time. The addition of propranolol (13 mumol/l) at different times after ISNA did not influence the rate of lipolysis, although it resulted in a decrease in the tissue level of cAMP. There was a positive correlation between the maximal increase in tissue cAMP and the rate of lipolysis in adipose tissue exposed to ISNA, both in individual experiments and in a group of 23 persons. No correlation was found between the rate of lipolysis and the tissue level of cAMP in adipose tissue incubated under basal conditions. The findings are compatible with the theory that the beta-adrenergic-induced lipolysis by human adipose tissue is a function of the maximal rise in the concentration of tissue cAMP. It is concluded that this peak level of cAMP represents single compartment of the nucleotide.
人体皮下脂肪组织在有无异丙肾上腺素（ISNA）的情况下进行了体外培养。在不同的培养时间段后，测量了环磷酸腺苷（cAMP）的组织浓度以及甘油向培养液中的释放量。在存在ISNA（6×10⁻⁵mol/L）的情况下，cAMP的组织浓度在大约10分钟后达到峰值，然后下降到明显低于培养开始时的水平。相比之下，ISNA诱导的脂解速率是培养时间的线性函数。在ISNA作用后的不同时间添加普萘洛尔（13μmol/L），虽然导致cAMP的组织水平降低，但并不影响脂解速率。在个体实验和一组23名受试者中，暴露于ISNA的脂肪组织中，组织cAMP的最大增加量与脂解速率之间均呈正相关。在基础条件下培养的脂肪组织中，未发现脂解速率与cAMP的组织水平之间存在相关性。这些发现与以下理论相符，即人体脂肪组织中β-肾上腺素能诱导的脂解作用是组织cAMP浓度最大升高的函数。得出的结论是，cAMP的这一峰值水平代表了核苷酸的单一区室。
