Arner P, Ostman J
J Lipid Res. 1978 Jul;19(5):613-8.
The relationship between mean fat cell size, maximal tissue cyclic AMP concentration, and glycerol release was investigated in human subcutaneous adipose tissue incubated in vitro with or without isoprenaline or noradrenaline added at maximal effective concentrations. Basal and stimulated glycerol release and cyclic AMP concentration were each related to the fat cell size. Whether or not the phosphodiesterase inhibitor theophylline was present in the incubation system, basal and noradrenaline-induced cyclic AMP levels were significantly correlated with the fat cell size. The noradrenaline-induced cyclic AMP levels resulted in twice as rapid glycerol release as could be expected from the basal ratio between glycerol release and cyclic AMP. Furthermore, both basal and noradrenaline-induced glycerol release in relation to the cyclic AMP levels were more rapid in enlarge fat cells. It is concluded that basal and catecholamine-induced production of cyclic AMP is related to the fat cell size and that a quantitative relationship exists between rate of lipolysis and maximal tissue levels of cyclic AMP in human adipose tissue. Basal and noradrenaline-induced lipolysis are probably regulated by different mechanisms and the lipolytic sensitivity to cyclic AMP seems increased in large fat cells.
在体外培养的人皮下脂肪组织中，添加或不添加最大有效浓度的异丙肾上腺素或去甲肾上腺素，研究平均脂肪细胞大小、最大组织环磷酸腺苷（cAMP）浓度与甘油释放之间的关系。基础和刺激后的甘油释放以及cAMP浓度均与脂肪细胞大小相关。无论孵育系统中是否存在磷酸二酯酶抑制剂茶碱，基础和去甲肾上腺素诱导的cAMP水平均与脂肪细胞大小显著相关。去甲肾上腺素诱导的cAMP水平导致甘油释放速度是根据基础甘油释放与cAMP之间的比例所预期速度的两倍。此外，在增大的脂肪细胞中，基础和去甲肾上腺素诱导的甘油释放相对于cAMP水平都更快。结论是，基础和儿茶酚胺诱导的cAMP产生与脂肪细胞大小相关，并且在人脂肪组织中，脂解速率与最大组织cAMP水平之间存在定量关系。基础和去甲肾上腺素诱导的脂解可能受不同机制调节，并且大脂肪细胞对cAMP的脂解敏感性似乎增加。
