用于测定氨基酚的酶循环电极比较：一种高灵敏度利钠肽检测系统的开发。

Comparison of enzymatic recycling electrodes for measuring aminophenol: development of a highly sensitive natriuretic peptide assay system.

作者信息

Mie Yasuhiro, Kowata Keiko, Hirano Yu, Niwa Osamu, Mizutani Fumio

机构信息

Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan.

出版信息

Anal Sci. 2008 May;24(5):577-82. doi: 10.2116/analsci.24.577.

DOI:10.2116/analsci.24.577

PMID:18469461

Abstract

Several redox enzymes were examined for enzymatic/electrochemical-recycling systems in order to measure p-aminophenol (PAP) with high sensitivity. Glucose oxidase (GOD) and diaphorase (DI) worked well as catalysts for recycling electrode systems: these enzymes effectively reduced p-iminoquinone (PIQ), the electrochemically-oxidized form of PAP, and caused an enhancement in the electrochemical signals (anodic currents in the voltammogram and amperogram) by approximately 100 fold. The lower detection limits for PAP were estimated to be 50 nM with the GOD system and 2 nM with the DI system. We combined the enzymatic-recycling electrode using DI with an enzyme immunoassay system to measure atrial natriuretic peptide (ANP), an important marker peptide hormone involved in heart diseases. ANPs from serum samples at ppt-levels were determined appropriately using the present assay system.

摘要

为了高灵敏度地测定对氨基苯酚（PAP），对几种氧化还原酶用于酶促/电化学循环系统进行了研究。葡萄糖氧化酶（GOD）和心肌黄酶（DI）作为循环电极系统的催化剂效果良好：这些酶有效地还原了PAP的电化学氧化形式对亚氨基醌（PIQ），并使电化学信号（伏安图和安培图中的阳极电流）增强了约100倍。使用GOD系统时，PAP的检测下限估计为50 nM，使用DI系统时为2 nM。我们将使用DI的酶促循环电极与酶免疫分析系统相结合，以测量心房利钠肽（ANP），这是一种参与心脏病的重要标记肽激素。使用本检测系统可适当测定血清样品中ppt水平的ANP。