Genome. 1996 Apr;39(2):379-94. doi: 10.1139/g96-049.
Barley (Hordeum vulgare L.) is one of the most extensively studied food crops in recent molecular research. More than 1000 molecular markers have been located on the barley genome by using five independent populations. For the present study, four segregation data sets, 'Proctor' x 'Nudinka', 'Igri' x 'Franka', 'Steptoe' x 'Morex', and 'Harrington' x TR306, were downloaded from the publicly available GrainGenes databank. Since 22% of the markers are common to at least two of the independent data sets, we were able to establish an integrated map using the computer package JOINMAP v2.0. The integrated map contains 898 markers, covers 1060 cM, and removes many large gaps present in the individual maps. Comparison of the integrated map with the individual maps revealed that the overall linear order of markers is in good agreement and that the integrated map is consistent with the component maps. No significant reordering of markers was found. This conservative property of the barley genome makes the integrated map reliable and successful. Except for chromosome 7 (5H), marker clustering was observed in the centromeric regions, probably owing to the centromeric suppression of recombination. Based on this integrated map, geneticists and breeders can choose their favourite markers in any region of interest of the barley genome. Key words : Hordeum vulgare, RFLP, integrated map.
大麦（Hordeum vulgare L.）是近年来分子研究中研究最多的粮食作物之一。通过使用五个独立群体，已经在大麦基因组上定位了超过 1000 个分子标记。在本研究中，从公开可用的 GrainGenes 数据库下载了四个分离数据集，'Proctor' x 'Nudinka'、'Igri' x 'Franka'、'Steptoe' x 'Morex'和'Harrington' x TR306。由于 22%的标记至少与两个独立数据集共有，因此我们能够使用计算机程序包 JOINMAP v2.0 建立一个综合图谱。综合图谱包含 898 个标记，覆盖 1060 cM，并消除了个别图谱中存在的许多大间隙。综合图谱与个别图谱的比较表明，标记的整体线性顺序非常一致，并且综合图谱与组成图谱一致。没有发现标记的明显重排。大麦基因组的这种保守特性使得综合图谱可靠且成功。除了 7 号染色体（5H）外，在着丝粒区域观察到标记聚类，这可能是由于着丝粒抑制了重组。基于这个综合图谱，遗传学家和育种家可以在大麦基因组的任何感兴趣区域选择他们喜欢的标记。关键词：大麦，RFLP，综合图谱。
