Department of Plant Production and Forest Science, University of Lleida, Lleida, Spain.
Theor Appl Genet. 2011 Mar;122(5):1029-37. doi: 10.1007/s00122-010-1507-2. Epub 2010 Dec 14.
This paper is concerned with a novel statistical-genetic approach for the construction of linkage maps in populations obtained from reciprocal translocation heterozygotes of barley (Hordeum vulgare L.). Using standard linkage analysis, translocations usually lead to 'pseudo-linkage': the mixing up of markers from the chromosomes involved in the translocation into a single linkage group. Close to the translocation breakpoints recombination is severely suppressed and, as a consequence, ordering markers in those regions is not feasible. The novel strategy presented in this paper is based on (1) disentangling the "pseudo-linkage" using principal coordinate analysis, (2) separating individuals into translocated types and normal types and (3) separating markers into those close to and those more distant from the translocation breakpoints. The methods make use of a consensus map of the species involved. The final product consists of integrated linkage maps of the distal parts of the chromosomes involved in the translocation.
本文提出了一种新的统计遗传学方法，用于构建来自大麦（Hordeum vulgare L.）相互易位杂合体群体的连锁图谱。在使用标准连锁分析时，易位通常会导致“假连锁”：将来自易位涉及的染色体的标记混合到单个连锁群中。在易位断点附近，重组受到严重抑制，因此，在这些区域对标记进行排序是不可行的。本文提出的新策略基于（1）使用主坐标分析解开“假连锁”，（2）将个体分为易位型和正常型，以及（3）将标记分为靠近和远离易位断点的标记。该方法利用了所涉及物种的共识图谱。最终产物包括所涉及易位染色体远侧部分的整合连锁图谱。
