Genome. 1995 Apr;38(2):368-80. doi: 10.1139/g95-048.
A molecular linkage map of cultivated oat composed of 561 loci has been developed using 71 recombinant inbred lines from a cross between Avena byzantina cv. Kanota and A. sativa cv. Ogle. The loci are mainly restriction fragment length polymorphisms detected by oat cDNA clones from leaf, endosperm, and root tissue, as well as by barley leaf cDNA clones. The loci form 38 linkage groups ranging in size from 0.0 to 122.1 cM (mean, 39 cM) and consist of 2-51 loci each (mean, 14). Twenty-nine loci remain unlinked. The current map size is 1482 cM and the total size, on the basis of the number of unlinked loci, is estimated to be 2932.0 cM. This indicates that this map covers at least 50% of the cultivated oat genome. Comparisons with an A-genome diploid oat map and between linkage groups exhibiting homoeology to each other indicate that several major chromosomal rearrangements exist in cultivated oat. This map provides a tool for marker-assisted selection, quantitative trait loci analyses, and studies of genome organization in oat.
利用金燕麦 cv. 卡诺塔和 A. sativa cv. 奥格尔杂交产生的 71 个重组自交系，我们构建了一份由 561 个基因座组成的栽培燕麦分子连锁图谱。这些基因座主要是通过燕麦叶片、胚乳和根组织的 cDNA 克隆以及大麦叶片 cDNA 克隆检测到的限制性片段长度多态性。这些基因座形成 38 个连锁群，大小从 0.0 到 122.1cM（平均值为 39cM），每个连锁群包含 2-51 个基因座（平均值为 14 个）。有 29 个基因座仍然没有连锁。目前的图谱大小为 1482cM，根据未连锁基因座的数量，总大小估计为 2932.0cM。这表明该图谱至少覆盖了栽培燕麦基因组的 50%。与 A 基因组二倍体燕麦图谱的比较以及彼此同源的连锁群之间的比较表明，栽培燕麦中存在几种主要的染色体重排。该图谱为标记辅助选择、数量性状位点分析以及燕麦基因组组织研究提供了工具。
