Portyanko V A, Chen G, Rines H W, Phillips R L, Leonard K J, Ochocki G E, Stuthman D D
Department of Plant Biology, University of Minnesota, St. Paul, 55108, USA.
Theor Appl Genet. 2005 Jul;111(2):313-24. doi: 10.1007/s00122-005-2024-6. Epub 2005 May 26.
To facilitate the detection of quantitative trait loci (QTLs) for partial resistance to oat crown rust, Puccinia coronata f. sp. avenae Eriks., a genetic map was generated in a population of 158 F(6)-derived oat recombinant inbred lines from a cross of a partial resistance line MN841801-1 by a susceptible cultivar selection 'Noble-2'. The map, developed using 230 marker loci, mostly restriction fragment length polymorphism and amplified fragment length polymorphism markers, spanned 1,509 cM (Haldane) arranged into 30 linkage groups of 2-18 markers each. Four consistently detected major QTLs for partial rust resistance, Prq1a, Prq1b, Prq2, and Prq7, and three minor QTLs, Prq3, Prq5, and Prq6, were found in tests involving three field and two greenhouse environments. In addition, two major QTLs for flowering time, Ftq1 and Ftq7, and five weaker QTLs, Ftq2, Ftq3, Ftq4, Ftq5, and Ftq6, were revealed. Overlapping of the map segments of Ftq1 and Prq1 and of Ftq7 and Prq7 suggested either linkage between the flowering time QTLs and resistance QTLs or a pleiotropic effect of the Ftq QTLs on rust resistance. Relatively low heritability estimates (0.30) obtained for partial resistance to crown rust in the field indicate a potential value for marker-assisted selection.
为便于检测燕麦对冠锈病部分抗性的数量性状基因座（QTL），针对燕麦冠锈病菌（Puccinia coronata f. sp. avenae Eriks.），利用一个由158个F(6)衍生的燕麦重组自交系群体构建了遗传图谱，该群体源自部分抗性品系MN841801 - 1与感病品种‘Noble - 2’的杂交。该图谱使用230个标记位点构建而成，主要是限制性片段长度多态性和扩增片段长度多态性标记，跨度为1509厘摩（霍尔丹），排列成30个连锁群，每个连锁群有2 - 18个标记。在涉及三个田间和两个温室环境的试验中，发现了四个一致检测到的对锈病部分抗性的主要QTL，即Prq1a、Prq1b、Prq2和Prq7，以及三个次要QTL，Prq3、Prq5和Prq6。此外，还发现了两个控制开花时间的主要QTL，Ftq1和Ftq7，以及五个较弱的QTL，Ftq2、Ftq3、Ftq4、Ftq5和Ftq6。Ftq1和Prq1以及Ftq7和Prq7的图谱区段重叠，表明开花时间QTL与抗性QTL之间存在连锁关系，或者Ftq QTL对锈病抗性具有多效性。田间对冠锈病部分抗性获得的相对较低的遗传力估计值（0.30）表明标记辅助选择具有潜在价值。
