氧化应激对红发夫酵母（隐甲藻）类胡萝卜素色素生产的影响。

Effects of oxidative stress on the production of carotenoid pigments byPhaffia rhodozyma (Xanthophyllomyces dendrorhous).

机构信息

Planta Piloto de Procesos Industriales Microbiologicos, Avda Belgrano y Pje. Caseros, 4000, San Miguel de Tucumán, Argentina,

出版信息

Folia Microbiol (Praha). 1998;43(2):173-6. doi: 10.1007/BF02816505.

DOI:10.1007/BF02816505

PMID:18470490

Abstract

The resistance to killing by free radicals of two mutants ofPhaffia rhodozyma was determined. Mutant 5-7 did not produce astaxanthin but produced beta-carotene, while mutant 3-4 did not produce any carotenoid pigments. The resistance of mutant 5-7 was the same as that of the wild type but mutant 3-4 was rapidly killed. Carotenoid pigments increased the resistance to killing by free radicals. We investigated the effects of free radicals, generated by H(2)O(2) and Fe(2+) added to the medium, on wild-type cells and mutants ofP. rhodozyma. Unpigmented mutants of basidiomycetous yeasts (Rhodotorula spp. and others) are more susceptible to killing by UV-irradiation than the pigmented, wild-type strains. Therefore, we investigated the effect of free radicals on a similar basidiomycetous yeast,P. rhodozyma, a species of economic importance, in the biological production of astaxanthin.

摘要

测定了两种粘红酵母（Phaffia rhodozyma）突变体对自由基杀灭的抗性。突变体 5-7 不产生虾青素，但产生β-胡萝卜素，而突变体 3-4 不产生任何类胡萝卜素色素。突变体 5-7 的抗性与野生型相同，但突变体 3-4 被迅速杀死。类胡萝卜素色素增加了对自由基杀灭的抗性。我们研究了由 H(2)O(2)和 Fe(2+)添加到培养基中产生的自由基对粘红酵母野生型细胞和突变体的影响。担子菌酵母（红酵母属和其他属）的非色素突变体比色素野生型菌株更容易被紫外线照射杀死。因此，我们研究了自由基对一种类似的担子菌酵母，即具有经济重要性的虾青素生物生产物种粘红酵母的影响。