Villa L L, Schlegel R
Ludwig Institute for Cancer Research, Sao Paulo, Brazil.
Virology. 1991 Mar;181(1):374-7. doi: 10.1016/0042-6822(91)90507-8.
Homologous, subgenomic fragments of the viral LCR and E6/E7 transforming genes of HPV-18 and HPV-16 were amplified from several primary cervical, penile, and vulvar tumors and cloned into a pUC-18-derived vector. When assayed by a quantitative transformation assay using primary human keratinocytes, the subgenomic regions of HPV-16 and HPV-18 exhibited transforming activities similar to that of the full-length, prototype HPV genomes. More importantly, the HPV-18 LCR-E6-E7 region was approximately 10- to 50-fold more active than that of HPV-16. These studies demonstrate (1) that the transforming activity differences previously observed between prototype HPV-16 and HPV-18 map to the LCR-E6-E7 region, and (2) that individual and independent isolates of HPV-16 and HPV-18 exhibit consistent differences in transforming potential, even when isolated from different anatomic sites.
从多个原发性宫颈癌、阴茎癌和外阴癌肿瘤中扩增出HPV - 18和HPV - 16病毒长控制区（LCR）及E6/E7转化基因的同源亚基因组片段，并克隆到源自pUC - 18的载体中。当使用原代人角质形成细胞通过定量转化试验进行检测时，HPV - 16和HPV - 18的亚基因组区域表现出与全长原型HPV基因组相似的转化活性。更重要的是，HPV - 18的LCR - E6 - E7区域的活性比HPV - 16高约10至50倍。这些研究表明：（1）先前在原型HPV - 16和HPV - 18之间观察到的转化活性差异定位于LCR - E6 - E7区域；（2）HPV - 16和HPV - 18的单个独立分离株在转化潜能上表现出一致的差异，即使是从不同解剖部位分离得到的。
