中国新疆地区宫颈感染女性人乳头瘤病毒 16 型 E1 和 E2 的遗传变异分析。
Analysis of genetic variation in human papillomavirus type 16 E1 and E2 in women with cervical infection in Xinjiang, China.
机构信息
Department of Biochemistry and Molecular Biology, School of Medicine, Xinjiang Endemic and Ethnic Disease and Education Ministry Key Laboratory, Shihezi University, Shihezi, 832002, Xinjiang, People's Republic of China.
School of Science, Technology and Engineering, Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, QLD, 4556, Australia.
出版信息
BMC Med Genomics. 2021 Nov 12;14(1):268. doi: 10.1186/s12920-021-01120-9.
BACKGROUND
Xinjiang is one of the regions with a high incidence of cervical cancer, and the genetic variation of human papillomavirus may increase its ability to infect the human body and enhance virus-mediated immune escape ability.
METHODS
Sanger sequencing of the HPV16 genome from 165 samples positive for HPV16 infection and phylogenetic analysis of the E1 and E2 genes revealed the gene polymorphism of HPV16 in Xinjiang.
RESULTS
The results showed that there were 109 samples with variations in HPV16 E1, 48 sites with nucleotide variations (19 missense variations and 29 synonymous variations), and 91 samples with variations in HPV16 E2, 25 sites with nucleotide variations (20 missense variations and five synonymous variations).
CONCLUSIONS
From the phylogenetic tree results, 149 samples were of the European variant and 16 samples were of the Asian variant. No African or North American/Asian variant types were found.
背景
新疆是宫颈癌高发地区之一，人乳头瘤病毒（HPV）的遗传变异可能会增加其感染人体的能力，并增强病毒介导的免疫逃逸能力。
方法
对 165 例 HPV16 感染阳性样本进行 HPV16 基因组的 Sanger 测序和 E1、E2 基因的系统进化分析，揭示新疆 HPV16 的基因多态性。
结果
结果显示 HPV16 E1 有 109 个样本发生变异，48 个核苷酸变异（19 个错义变异和 29 个同义变异），HPV16 E2 有 91 个样本发生变异，25 个核苷酸变异（20 个错义变异和 5 个同义变异）。
结论
从系统进化树结果来看，149 个样本为欧洲变异株，16 个样本为亚洲变异株，未发现非洲或北美/亚洲变异株类型。
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