Department of Biochemical Pharmacology, The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, UK.
Mediators Inflamm. 1997;6(5-6):363-7. doi: 10.1080/09629359791505.
Overnight incubation of human eosinophils (Eøs) with the glucocorticoid hormone dexamethasone (DEX; 0.1 microM) resulted in lower expression of the CD11b, but not CD49d, antigen on their plasma membrane, as assessed by flow cytometry. DEX produced a consistent inhibitory effect (ranging from 16% to 20%) when tested at a concentration of 0.1 microM. Eø stimulation with 100 ng/ml eotaxin produced an increase in CD11b (+26%), but not CD11c, levels and concomitantly a reduction (-25%) on CD62L expression. The inhibition exerted by DEX upon CD11b levels was also evident following eotaxin upregulation, with a degree of inhibition similar to that seen on basal levels. These data highlight a novel mechanism of action by which glucocorticoid hormones may be effective in reducing Eø accumulation during allergic inflammation in man.
地塞米松（DEX；0.1μM）孵育过夜可降低人嗜酸性粒细胞（Eø）细胞膜上 CD11b 的表达，但不影响 CD49d 抗原，流式细胞术检测结果表明。DEX 在 0.1μM 浓度下测试时具有一致的抑制作用（范围为 16％至 20％）。100ng/ml 趋化因子 eotaxin 刺激可增加 CD11b（+26％），但不增加 CD11c 水平，同时降低 CD62L 表达（-25％）。地塞米松对 CD11b 水平的抑制作用在 eotaxin 上调后也很明显，抑制程度与基础水平相似。这些数据突出了糖皮质激素可能通过一种新的作用机制在人类过敏炎症期间减少 Eø 积累。
