地塞米松对酵母聚糖和细胞因子诱导豚鼠肺泡巨噬细胞 TXB2 释放的差异反应。
Differential response to dexamethasone on the TXB2 release in guinea-pig alveolar macrophages induced by zymosan and cytokines.
机构信息
Departamento de Medicina, Farmacología, Facultad de Medicina, Julián Clavería s/n, Oviedo 33006, Spain.
出版信息
Mediators Inflamm. 1997;6(5-6):375-80. doi: 10.1080/09629359791523.
Glucocorticosteroids reduce the production of inflammatory mediators but this effect may depend on the stimulus. We have compared the time course of the effect of dexamethasone on the thromboxane B2 (TXB2) release induced by cytokine stimulation and zymosan in guinea-pig alveolar macrophages. Interleukin-1beta (IL-1beta), tumour necrosis factor-alpha (TNF-alpha) and opsonized zymosan (OZ), all stimulate TXB2 release. High concentrations of dexamethasone (1-10 microM) inhibit the TXB2 production induced by both cytokines and OZ, but the time course of this response is different. Four hours of incubation with dexamethasone reduce the basal TXB2 release and that induced by IL-1beta and TNF-alpha, but do not modify the TXB2 release induced by OZ. However, this stimulus was reduced after 24 h incubation. Our results suggest that the antiinflammatory activity of glucocorticosteroids shows some dependence on stimulus and, therefore, may have more than one mechanism involved.
糖皮质激素可减少炎症介质的产生，但这种作用可能取决于刺激物。我们比较了地塞米松对细胞因子刺激和酵母聚糖诱导豚鼠肺泡巨噬细胞释放血栓素 B2（TXB2）的时间过程的影响。白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和调理酵母聚糖（OZ）均刺激 TXB2 释放。高浓度地塞米松（1-10 μM）抑制两种细胞因子和 OZ 诱导的 TXB2 产生，但这种反应的时间过程不同。地塞米松孵育 4 小时可降低基础 TXB2 释放和 IL-1β和 TNF-α诱导的 TXB2 释放，但不改变 OZ 诱导的 TXB2 释放。然而，这种刺激在 24 小时孵育后减少。我们的结果表明，糖皮质激素的抗炎活性表现出对刺激物的一定依赖性，因此可能涉及多种机制。
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