Albrightson C R, Baenziger N L, Needleman P
J Immunol. 1985 Sep;135(3):1872-7.
Incubation of cultured human umbilical vein endothelial cells with factors derived from human peripheral blood mononuclear cells (MNCF) or adherent monocytes (AMF) resulted in concentration-and time-dependent increases in prostacyclin and prostaglandin E2 (PGE2) production. MNCF and AMF also stimulated prostacyclin and PGE2 biosynthesis in cultured human arterial smooth muscle cells and human dermal fibroblasts. The effect of these monokines on endothelial cells and fibroblasts was mimicked by treatment with purified human interleukin 1 (IL 1). Mononuclear cell-conditioned medium subjected to gel filtration yielded fractions (Mr 12,000 to 18,000 daltons) which simultaneously contained endothelial cell and fibroblast prostaglandin-stimulating activity and IL 1 activity. Therefore, monokines, specifically IL 1, appear to serve as chemical mediators of the interaction between monocytes and vascular cells as would occur in blood vessel injury, inflammation, and atherosclerosis.
用人外周血单核细胞衍生因子（MNCF）或贴壁单核细胞（AMF）培养人脐静脉内皮细胞，会导致前列环素和前列腺素E2（PGE2）生成量呈浓度和时间依赖性增加。MNCF和AMF还能刺激培养的人动脉平滑肌细胞和人皮肤成纤维细胞中前列环素和PGE2的生物合成。用纯化的人白细胞介素1（IL 1）处理可模拟这些单核因子对内皮细胞和成纤维细胞的作用。对单核细胞条件培养基进行凝胶过滤得到的组分（相对分子质量12,000至18,000道尔顿）同时含有内皮细胞和成纤维细胞前列腺素刺激活性以及IL 1活性。因此，单核因子，特别是IL 1，似乎可作为单核细胞与血管细胞之间相互作用的化学介质，这种相互作用会在血管损伤、炎症和动脉粥样硬化中发生。
