血小板激活因子作用于豚鼠眼后，血-泪屏障对荧光素和白蛋白的通透性。
Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig.
机构信息
Department of Ophthalmology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The Netherlands.
出版信息
Mediators Inflamm. 1997;6(5-6):381-3. doi: 10.1080/09629359791532.
One of the inflammatory responses of the eye to local application of platelet-activating factor (PAF) is oedema of the conjunctiva, caused by extravasation of plasma. Aim of the study was to investigate if fluorescein would leak from the blood into the tears together with plasma protein after application of PAF to the eye. Fluorescein was given intraperitoneally 30 min prior to application of 25 microl of 0.1% solution of PAF. Thirty min after PAF the tear film was collected by washing the surface of the eye with 25 microl of phosphate buffered saline (PBS). Fluorescein in eye washings and in plasma was measured by fluorophotometry and albumin by immunodiffusion. Both fluorescein and albumin appeared in a related fashion in tears, being absent in washings of placebo-treated control eyes. Extravasation of fluorescein can be used as a measure for plasma leakage in the conjunctiva with the advantage over the Evans Blue method that the former is a non-invasive method.
眼部局部应用血小板激活因子（PAF）会引起结膜水肿，这是一种由血浆外渗引起的炎症反应。本研究旨在探讨 PAF 作用于眼部后，荧光素是否会与血浆蛋白一起从血液漏入泪液。在应用 0.1% PAF 溶液 25 微升之前 30 分钟，将荧光素腹膜内给药。在 PAF 作用 30 分钟后，用 25 微升磷酸盐缓冲盐水（PBS）冲洗眼表面收集泪膜。通过荧光光度法测量眼洗液和血浆中的荧光素，通过免疫扩散法测量白蛋白。荧光素和白蛋白在泪液中以相关的方式出现，而在安慰剂治疗的对照眼的洗液中则不存在。荧光素外渗可作为结膜血浆渗漏的衡量指标，与 Evans Blue 法相比，前者具有非侵入性的优点。
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